IL-11对中子辐射小鼠肠道损伤保护作用的定量病理研究  

Quantitative study on the effect of IL-11 on the intestinal pathological changes of mice after neutron irradiation

作　　者：王瑞娟[1] 彭瑞云[1] 韩瑞刚[1] 高亚兵[1] 马俊杰[1] 徐新萍[1] 王水明[1] 李杨[1] 王德文[1] 

机构地区：[1]军事医学科学院放射与辐射医学研究所,北京100850

出　　处：《中国体视学与图像分析》2006年第2期139-143,共5页Chinese Journal of Stereology and Image Analysis

基　　金：国家自然科学基金资助课题(No.30370438)

摘　　要：目的定量研究IL-11对中子照射后小鼠肠道损伤的保护作用。方法4.0 Gy中子全身照射136只BALB/C二级小鼠,于照前和照后注射600μg/kg的rhIL-11(每日1次,连用3 d),并于照后6 h、1 d、2 d和5 d活杀取材,采用光镜和图像分析技术研究IL-11对中子照射后肠道病理形态、肠绒毛高度和隐窝细胞数的影响。结果4.0 Gy中子照射后,肠隐窝细胞核肿胀、浓缩及核碎片形成,再生微弱;照前和照后给予IL-11,肠绒毛高度增加、绒毛上皮和隐窝数量增多,有较多新生的肠隐窝,与单纯照射组比较,差异显著(p<0.05或0.01)。结论应用IL-11可减轻中子辐射肠道损伤的程度,增加存活隐窝细胞数量和绒毛高度,并促进其再生,有利于肠粘膜恢复。Objectives To study the protective effect of IL-11 on the intestine after neutron irradiation. Methods 136 BALB/C mice were irradiated by 4.0 Gy neutron and injected subcutaneously with rh IL-11 at a dose of 600 μg/kg body weight once per day for 3 days prior to or after irradiation. The mice were killed at 6 h, 1 d, 2 d, 3 d and 5 d after irradiation. Effects of IL-11 on the intestinal histopathological changes, villus length and number of cryptal cells were observed by means of microscopy and image analysis. Results After 4.0 Gy neutron irradiation, the cryptal cell neucleus showed swelling, pyknosis and karyorrhexis. Very slight regeneration was observed. Compared with those irradiated mice without IL-11, IL-11-treated mice showed higher villus and more epithelial and cryptal cell （p 〈0.05 or 0.01 ）. Conclusions IL-11 administration reduced the severity of intestinal damages, increased the number of cryptal ceils and accelerated recovery of intestinal mucosa.

关 键 词：中子 肠道 病理 IL-11 

分 类 号：R818[医药卫生—放射医学]