甲基强的松龙对体外培养许旺氏细胞的作用  

In vitro effect of methylprednisolone on the proliferation of Schwann cells

作　　者：党育[1] 姜保国[1] 张培训[1] 张殿英[1] 张宏波[1] 

机构地区：[1]北京大学人民医院创伤骨科,北京市100044

出　　处：《中国脊柱脊髓杂志》2006年第B07期50-52,共3页Chinese Journal of Spine and Spinal Cord

基　　金：神经损伤修复和功能重建的应用基础研究(973项目)编号2003CB515306

摘　　要：目的:探讨甲基强的松龙(MP)对体外培养许旺氏细胞的作用。方法:取成年SD大鼠坐骨神经以植块法分离纯化许旺氏细胞,将纯化后的细胞培养2周后分为5组移入96孔板,A、B、C组为MP组(按加入药物浓度不同分组20μg/ml,2μg/ml,0.2μg/ml),D组为神经生长因子(NGF)组(药物浓度0.1μg/ml),E组为对照组。继续培养9d后倒置显微镜下观察细胞生长情况并以MTT法测定各组许旺氏细胞的存活与增殖能力。结果:倒置显微镜下观察小剂量MP组及NGF组细胞生长优于其他各组。570nm波长分光光度计测定A值,小剂量(0.2μg/ml)MP组为0.376±0.079,中剂量(2μg/ml)MP组0.286±0.116,大剂量(20μg/ml)MP组0.280±0.086,NGF组0.320±0.121,对照组0.252±0.115,小剂量MP组明显优于大剂量MP组(P<0.05),同时优于对照组(P<0.05),余各组间均无明显统计学差异。结论:小剂量MP能够促进体外培养许旺氏细胞增殖,从而促进周围神经损伤的修复,细胞培养用药浓度为0.2μg/ml。MP浓度过大(相当于临床冲击剂量的血峰浓度)将抑制细胞增殖。Objective：To study the effect of methylprednisolone （MP） on the proliferation of Schwann cell cultured in vitro.Method：Schwann cells were harvested and purified from sciatic nerve of adult SD rats.After culturing for 2 weeks,they were divided into 5 groups：the methylprednisolone groups （3 groups according to different concentration of methylprednisolone）,NGF group and control group.The cell proliferation was observed through the phase contrast microscope.After culturing for 9 days,MTT assay was employed to observe the growth of Schwann cells.The samples were measured at wave length 570nm to compare the A values.Result： When methylprednisolone was 2μg/ml,no obvious proliferation of Schwann cell was found.The A value of 0.2μg/ml methylprednisolone group was 0.376±0.079,20μg/ml methylprednisolone group was 0.280±0.086 （P〈 0.05 ） and control group was 0.252±0.115 （P〈0.05）.Which had significant differences.Conclusion： Methylprednisolone can accelerate Schwann cell proliferation in vitro.While overdose can reverse this process.

关 键 词：许旺氏细胞 周围神经 甲基强的松龙 细胞培养 MTT法 

分 类 号：Q813.1[生物学—生物工程] R977.1[医药卫生—药品]