Molecular Mapping of Two Novel Stripe Rust Resistant Genes YrTp1 and YrTp2 in A-3 Derived from Triticum aestivum × Thinopyrum ponticum  

作　　者：YIN Xue-gui SHANG Xun-wu PANG Bin-shuang SONG Jian-rong CAO Shi-qin LI Jin-chang ZHANG Xue-yong 

机构地区：[1]Key Laboratory of Crop Germplasm ＆ Biotechnology, Ministry of Agriculture~Institute of Crop Science, Chinese Academy of Agricultural Sciences~The National Facility for Crop Gene Resources and Genetic Improvement, Beijing 100081, P.R.China [2]Agronomy College, Gansu Agricultural University, Lanzhou 730070, P.R.China [3]Tianshui Institute of Agricultural Sciences, Tianshui 741000, P.R.China [4]Institute of Plant Protection, Gansu Academy of Agricultural Sciences, Lanzhou 730070, P.R.China

出　　处：《Agricultural Sciences in China》2006年第7期483-490,共8页中国农业科学（英文版）

摘　　要：Loss of variety resistance to stripe rust （Puccinia striiformis Westend f. sp.tritici） is an important factor causing massive periodical epidemic of rust in wheat production. Creation and development of new races of rust pathogen have led to serious crisis of resistance loss in widely planted varieties. This has quickened the search for new resistance resources. Molecular marker could facilitate the identification of the location of novel genes. A line A-3 with high resistance （immune） to currently epidemic yellow rust races （CY29, 31, 32） was screened out in offspring of Triticum aestivura x Thinopyrum ponticum. Segregation in F2 and BC1 populations indicated that the resistance was controlled by two independent genes： one dominant and one recessive. SSR markers were employed to map the two resistant genes in the F2 and BC1 populations. A marker WMC477-167bp located on 2BS was linked to the dominant gene with genetic distance of 0.4 cM. Another marker WMC364-2os bp located on 7BS was linked to the recessive-resistant gene with genetic distance of 5.8 cM. The two genes identified in this paper might be two novel stripe rust resistant genes, which were temporarily designated as YrTpl and YrTp2, respectively. The tightly linking markers facilitate transfer of the two resistant genes into the new varieties to control epidemic of yellow rust.Loss of variety resistance to stripe rust （Puccinia striiformis Westend f. sp.tritici） is an important factor causing massive periodical epidemic of rust in wheat production. Creation and development of new races of rust pathogen have led to serious crisis of resistance loss in widely planted varieties. This has quickened the search for new resistance resources. Molecular marker could facilitate the identification of the location of novel genes. A line A-3 with high resistance （immune） to currently epidemic yellow rust races （CY29, 31, 32） was screened out in offspring of Triticum aestivura x Thinopyrum ponticum. Segregation in F2 and BC1 populations indicated that the resistance was controlled by two independent genes： one dominant and one recessive. SSR markers were employed to map the two resistant genes in the F2 and BC1 populations. A marker WMC477-167bp located on 2BS was linked to the dominant gene with genetic distance of 0.4 cM. Another marker WMC364-2os bp located on 7BS was linked to the recessive-resistant gene with genetic distance of 5.8 cM. The two genes identified in this paper might be two novel stripe rust resistant genes, which were temporarily designated as YrTpl and YrTp2, respectively. The tightly linking markers facilitate transfer of the two resistant genes into the new varieties to control epidemic of yellow rust.

关 键 词：WHEAT Thinopyrum ponticum stripe rust resistant gene SSR MAPPING 

分 类 号：S512.1[农业科学—作物学]