RP-HPLC直接测定普济痔疮栓中牛磺熊去氧胆酸含量  

Determination of Tauroursodeoxycholic Acid in Puji Suppository for Hemorrhoids by RP-HPLC

作　　者：王守箐[1] 

机构地区：[1]临沂师范学院,山东临沂276005

出　　处：《食品与药品》2006年第08A期52-53,共2页Food and Drug

摘　　要：目的建立HPLC测定普济痔疮栓中牛磺熊去氧胆酸含量的方法。方法样品经提取后用HPLC测定。色谱条件:C18柱。流动相:甲醇-水-冰醋酸(78:22:0.01)。流速1.0mL/min,柱温:室温;检测器为ELSD,漂移管温度105℃;雾化气体:空气;流速2.0L/min。结果本法线性关系良好(r=0.9982),牛磺熊去氧胆酸平均回收率为99.9%,RSD为1.03%。结论本法较原方法操作简便、重复性好,适合普济痔疮栓的含量控制。Objective To establish a HPLC method for the determination of tauroursodeoxycholic acid （TUDCA） in Puji Suppository for hemorrhoids. Methods Phenomenex C18 column was used at room temperature. The mobile phase consisted of methanol-water-glacial acetic acid （78：22：0.01） and the flow rate was 1.0 mL/min. The temperature of drift tube of ELSD detector was 105 ℃ and the nebulizer gas was air with the flow rate of 2.0 mL/min. Results TUDCA has a good linear relation （r=0.998 2）. The average recovery was 99.9% and RSD was 1.03%. Conclusion The method is simpler than the original standard and the repeatability is better. It is suitable for the quality control of TUDCA.

关 键 词：普济痔疮栓 牛磺熊去氧胆酸 高效液相色谱-蒸发光散射检测器 

分 类 号：Q657.7[生物学—生物物理学]