高效液相色谱法测定斑蝥中斑蝥素的含量  

Quantitative Determination of Cantharidin in Mylabris by HPLC

作　　者：周祥敏 邹丽[2] 

机构地区：[1]重庆市药品检验所,重庆400015 [2]四川省药品检验所,四川成都610036

出　　处：《食品与药品》2006年第08A期54-55,共2页Food and Drug

摘　　要：目的建立测定斑蝥中斑蝥素含量的高效液相色谱方法。方法以C18色谱柱为分析柱,乙腈-水(80:20)为流动相,检测波长为209nm。结果斑蝥素进样量在0.4012～2.0060范围内与峰面积呈线性关系,平均回收率为100.1%,RSD为2%。结论本方法准确可靠,能有效地控制斑蝥药材的质量。Objective To establish HPLC method to measure the content of cantharidin in Mylabris. Methods The condition was as follows： Kromacil C 18 （4.6 mm 50 mm, 5） column; the mobile phase consisted of methanol - water （80：20）, the detection wavelength was 254 nm. Result The calibration curve was liner over a range from 0.401 2 to 2.006 0. The average recovery rates （n=6） were 100.1% （RSD =2%）. Conclusion The method is correct, and can control the quality of the Mylabris.

关 键 词：斑蝥 斑蝥素 高效液相色谱法 

分 类 号：R384[医药卫生—医学寄生虫学]