DA、5-HT及其代谢产物HPLC-ECD检测方法的研究  

A HPLC-ECD Method to Study DA, 5-HT and their Metabolite

作　　者：杨威[1] 邓玉林[1] 徐敏[1] 

机构地区：[1]北京理工大学生命科学与技术学院,北京100081

出　　处：《现代科学仪器》2006年第3期44-46,共3页Modern Scientific Instruments

摘　　要：采用高效液相色谱结合库仑阵列多电极检测器体系(HPLC-ECD)建立了一套操作简便、高效、快速测定多巴胺(DA)、3,4-二羟基苯乙酸(DOPAC)、高香草酸(HVA)、5-羟色胺(5-HT)以及5-羟吲哚乙酸(5-HIAA)的方法,并探究了检测的灵敏性,浓度和响应因子之间的线性关系,系统的稳定性等。DA、DOPAC、HVA、5-HT及5-HIAA的相关系数分别为0.9964、0.9999、1、0.9989、0.9999;HVA和5-HIAA的检测限都低于0.5ng/mL,DA、DOPAC及5-HT的检测限均低于0.1ng/mL,各种物质的方法回收率为85.6%-107.4%。High performance liquid chromatography（HPLC-ECD）was used for measurement of the neurotrasmitters. The method of measuring DA, DOPAC, HVA, 5-HT and 5-HIAA was established. Systematic investigations of various factors involved in contributing to the reliability and reproducibility of the neurotrasmitters measurement were described. These factors include sensitivity of detection, limits of linear relationship between concentration and the response factor, stability of the system. The response factor of DA. DOPAC. HVA. 5-HT and 5-HIAA are respective 0.9964. 0.9999. 1. 0.9989 and 0.9999. The limits of detection of HVA and 5-HIAA are below 0.5ng/mL,and DA. DOPAC. 5-HT are below 0. 1ng/mL.

关 键 词：HPLC-ECD 神经递质 检测方法 

分 类 号：O657.7[理学—分析化学]