脱皮榆离体培养再生植株的研究  

PLANTLET FORMATION OF ULMUS LAMELLOSA COLTURED IN VITRO

作　　者：杜盛[1] 闫美杰[1] 

机构地区：[1]内蒙古林学院林学系,呼和浩特市郊区林业局

出　　处：《内蒙古林学院学报》1996年第4期15-17,共3页

基　　金：内蒙古教育厅资助项目

摘　　要：本文在过去研究的基础上首次获得了脱反榆试管苗，并移栽成活。采用脱皮榆实生幼苗的子叶、叶及顶芽为外植体，经愈伤组织或直接形成丛生芽。嫩梢在Ｍｓ＋０．２ｍｇ／ＬＩＢＡ的培养基上诱导产生不定根，填加ＰＧ及稀释无机盐用量利于不定根形成。ln vitro pl antlets of Ulmus lamellosa were produced under defined conditionsfrom explants of cotyledons,leaves and shoot tips from 2-to 4.-week-old seedlings.Axillaryand adventitious buds were induced from shoot explants and callus,respectively. Plantletswere regenerated by rooting excised shoots on agar-solidifed medinum containing sucrose,MSinorganic nutrients and IBA. Rooting could be enhanced by supplementing the medium withPG or lowering the macronutrient concentrations.Plantlets were then established in soil.

关 键 词：脱皮榆 组织培养 微体繁殖 

分 类 号：S792.190.4[农业科学—林木遗传育种]