严重烧伤早期大鼠心室乳头肌细胞内钾离子浓度及动作电位的变化  

Changes in intracellular K^+ concentration andaction potentials of myocardiocytes after early serious burn injury in rats

作　　者：李敏[1] 肖家思[1] 阎淑枝[1] 万子兵[1] 

机构地区：[1]第三军医大学生理教研室

出　　处：《中国病理生理杂志》1996年第6期619-622,共4页Chinese Journal of Pathophysiology

摘　　要：健康成年Ｗｉｓｔａｒ大鼠４０只，随机分为５组：正常对照组和烧伤后第１ｈ、３ｈ、８ｈ和２４ｈ组。应用Ｋ￣＋离子选择性微电极和普通玻璃微电极技术结合微型计算机进行分析的方法，观察严重烧伤（３５％ＴＢＳＡⅢ°烧伤）早期大鼠心室乳头肌细胞内Ｋ￣＋浓度和动作电位的变化。结果表明：与对照组相比：①大鼠烧伤后２４ｈ内心室肌细胞内Ｋ￣＋浓度、动作电位幅度、静息电位和零期最大去极化速率均显著降低，烧伤后８ｈ达最低值，２４ｈ有所恢复；②动作电位复极化时程ＡＰＤ＿（５０）和ＡＰＤ＿（９０）均在烧伤后３ｈ和８ｈ显著缩短，ＡＰＤ＿（１０）仅在烧伤后８ｈ明显缩短，２４ｈ三者均基本恢复。提示：烧伤早期主要影响心室肌细胞快钠通道和Ｋ￣＋通道，抑制Ｎａ￣＋内流，促进Ｋ￣＋外流。Forty Wistar rats were examined in this experiment.K￣+一ion selectivemicroelectrodes and conventional microelectrodes were used respectively to measure theintraeellular K ￣+oneentration([K+]i),resting potentials(RP), and action potentials (AP) ofventricular papillary muscle of control group and the 1h、 3h、 8h and 24h after burn injuredgroups(35% TBSA Ⅲ°burns).As compared with the control group,it was found that:①[K+]i、LRP、 APA and Vmax were all decreased significantly after early burn injury, and wasall down to the lowest level at 8h or so,then recovered gradually,②APD_(50) and APD_(90) wereshortened obviously at 3h and 8h,APD_(10) shortened only at 8h, and all recovered nearly tothe control level at 24h after burn injury,These results suggested that K+ outward currentwas accelerated,while Na+ inward current was diminished after early hurn injury.

关 键 词：灼伤 心肌 钾 动作电位 

分 类 号：R644.02[医药卫生—外科学]