琼脂糖微囊人工膜制备的实验研究  

EXPERIMENTAL STUDY OF PREPARATION OF AGAROSE BIOARTIFICIAL MICRO-CAPSULES

作　　者：于德民[1] 尹潍[1] 吴锐[1] 高伟[2] 姚康德[2] 

机构地区：[1]天津医科大学总医院内分泌科,300052 [2]天津大学材料系

出　　处：《中华内分泌代谢杂志》1996年第4期218-221,共4页Chinese Journal of Endocrinology and Metabolism

摘　　要：使用美国Sigma公司琼脂糖,国产琼脂糖以及国产琼脂糖加上其他合成高分子制备成的互穿聚合物网络(IPN),对分子量不同的4种物质(甲基橙、VitB_(12)、胰岛素、溶菌酶)进行了渗透性能的研究。结果表明,对分子量小于6 000的溶质,在3种膜中的渗透系数相近,都可实现其通透。而对于大分子溶质(14 300)的隔离作用上,IPN膜具有良好的免疫隔离性能,两种琼脂糖膜虽然对溶菌酶这样的大分子有一定的隔离作用,但效果远差于IPN膜。研制出的免疫隔离效果优异的琼脂糖和合成高分子互穿网络,基本达到移植用要求。对6只糖尿病大鼠施行腹腔内植入微囊化胰岛手术,2 000～3 000个/只,不用任何免疫抑制剂。移植后第4天血糖明显下降,第7天时血糖至正常范围,维持达30天。实验表明所制备的琼脂糖微囊具有较邓的免疫隔离作用,为今后使用微包囊猪胰岛移值治疗Ⅰ型糖尿病的临床研究奠定了基础。The permeability of three kinds of membranes, including imported agarose (Sigma), China-made agarose and interpenetrating polymer network (IPN) made of Chinese agarose mixed with other synthetic macromolecular compound, to four substances with different molecular weights (methyl orange, Vit B12 , insulin and lysozyme) was studied. The results revealed that the solutes with molecular weights below 6 000 dalton could permeate all kinds of membranes with similar coefficient of permeability. However, the immunoisolation of IPN for such macromolecular solute, as lysozyme (14 300 dalton) was much more effective than that of other kinds, which only had limited effect of immunoisolation. These results suggest that IPN with the characteristics of efficacious immunoisolation fulfills the requirements for transplantation. 2 000-3 000 microencapsulated islets were transplanted intraperitoneally in six diabetic rats without the use of immunosuppressive drugs. On the 4th post-operative day, blood sugar dropped significantly until the 7th day, when it was in normal range. The normal level of blood sugar had remained for 30 days. The microcapsules, made of IPN, possesses a better function of immunoisolation, which may lay a foundation of treating IDDM with encapsulated porcine islet grafting.

关 键 词：微囊 琼脂糖 糖尿病 

分 类 号：R587.105[医药卫生—内分泌] R944.9[医药卫生—内科学]