性激素及人前列腺生长因子对人前列腺间质成纤维细胞增殖的影响  

EFFECT OF SEX HORMONES AND HUMAN PROSTATIC GROWTH FACTOR ON THE PROLIFERATION OF CULTURED FIBROBLASTS DERIVED FROM HUMAN FETAL PROSTATE

作　　者：秦志杰[1] 林毓琴[1] 罗道春[1] 赵春华[1] 刘贤锡[1] 张虞毅[1] 

机构地区：[1]山东医科大学生化教研室,山东潍坊医学院生化教研室261042

出　　处：《中华内分泌代谢杂志》1996年第4期226-229,共4页Chinese Journal of Endocrinology and Metabolism

摘　　要：从人胎儿前列腺组织中分离培养了间质成纤维细胞,观察雄激素(DHT)、雌激素(E_2)及部分纯化的人前列腺生长因子(hPGF)对该细胞增殖的影响。结果发现:(1)DHT及hPGF对体外培养的人前列腺间质成纤维细胞均具有明显的促增殖作用,但前者表现为延迟反应,后者为即时效应;(2)雌激素对该细胞无刺激增殖作用;(3)E_2与DHT联合应用对该细胞增殖刺激作用也无明显影响。实验结果提示:DHT对人前列腺间质成纤维细胞具有间接的刺激增殖作用,可能通过刺激前列腺细胞自分泌或旁分泌作用而调节前列腺生长。Prostatic stromal fibroblasts were obtained from human fetal prostate and the effect of androgen (DHT) , estrogen (E2) , and human prostatic growth factor (hPGF) on the proliferation of the cultured fibroblasts was studied. The results showed that: 1) DHT and hPGF had significant stimulatory effect on proliferation of the cells; DHT had a delayed stimulatory effect, while hPGF had an immediate one. 2) E2 had no effect on proliferation of the cultured fibroblasts. 3) Used jointly, E2 and DHT had no stimulating effect on the proliferation of the fibroblasts. The results suggest that the effect of DHT on the proliferation of human prostatic stromal fibroblasts may be indirect. It possibly regulates the growth of prostate by stimulating autocrine or paracrine function of the prostatic cells.

关 键 词：性激素 生长因子 间质成纤维细胞 前列腺增生 

分 类 号：R697.320.2[医药卫生—泌尿科学]