不同分化程度的类成骨细胞对流体刺激的生物反应  

Biological responses of osteoblast-like cells in different maturation stages under shear flow

作　　者：杜大江[1] 高中礼[1] 刘光耀[1] 古川克子[2] 牛田多加志[2] 

机构地区：[1]吉林大学中日联谊医院骨科,长春130000 [2]东京大学工学部研究科

出　　处：《中国矫形外科杂志》2006年第15期1182-1184,共3页Orthopedic Journal of China

摘　　要：[目的]考察不同分化程度的骨组织细胞对流体刺激的生物反应。[方法]利用平行板流体刺激小室系统对以成骨分化诱导液诱导的不同分化程度类成骨细胞MC3T3-E1进行2 h流体刺激,剪切力为0.0 462 mN.s/cm2,24 h后收集样本,进行双链DNA和碱性磷酸酶测定。[结果]流体刺激24 h后,非诱导分化组ALP活性无明显变化,诱导分化4 d组ALP活性明显升高,诱导分化9 d组ALP活性明显降低。[结论]不同分化程度的类成骨细胞对流体刺激的反应不同,对骨的生长与重建有着重要意义。[ Objective] To sturdy the biological responses of osteoblast-like cells in different maturation stages under shear flow. [ Method] Flow chamber system was used to apply shear flow stimulation of 4. 62dyn · s/cm^2 for 2 hours on osteoblast-like cell MC333-E1 of different maturation stage induced by osteogenic media, and then samples for dsDNA content and ALP activity analysis were collected. [ Result ] On 24 hours after simulation, there was not obvious change of ALP activity with non-osteogenic inducing group, an increase was observed in the group of 4 days of differentiation, while an decrease in the group of 8 days of differentiation. [ Conclusion] Biological responses of osteoblast-like cells in different maturation stages under shear flow is quite different, and this may be an important role in bone growth and reconstruction.

关 键 词：机械刺激 成骨分化 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]