DSP基因编码区序列的多态性研究  

Nucleotide polymorphisms analysis of dentin sialoprotein

作　　者：王英[1] 张莹[1] 史俊南[1] 

机构地区：[1]西安第四军医大学口腔医院牙体科,710032

出　　处：《实用口腔医学杂志》2006年第4期443-446,共4页Journal of Practical Stomatology

摘　　要：目的:分析中国人群中牙本质涎蛋白基因编码区序列的多态性。方法:采用聚合酶链式反应-单链构象多态(PCR-SSCP)分析方法,并结合DNA直接测序方法对牙本质涎蛋白基因编码区的核苷酸序列进行分析。结果:在牙本质涎蛋白基因编码区序列中发现了3个单核苷酸多态(cSNP),其中2个为同义cSNP,编码的氨基酸未变,1个为非同义cSNP,编码的氨基酸分别为天冬氨酸和天冬酰氨。结论:中国人群中牙本质涎蛋白基因编码区序列中存在单核苷酸多态。Objective：To analyse the nucleotide polymorphism of dentin sialoprotein（DSP） coden region in Chinese people. Methods：The DSP segments were amplified by PCR;single-stranded conformation polymorphism（ SSCP） and DNA sequencing were employed to detect the nucleotide polymorphisms in DSP coden region. Results： Three single nucleotide polymorphisms（SNP） were found in DSP coden region,two were same sense SNPs resulting in no change of amino acid product,and one was missense SNP resulting in change of asparagine and aspartic acid. Conclusion：There are some SNPs existing in DSP coden region in Chinese people.

关 键 词：牙本质涎蛋白 单核苷酸多态 

分 类 号：Q987[生物学—遗传学]