血清蛋白质指纹图谱构建及对慢性移植物肾病病人的早期诊断意义  

Construction of protein fingerprint signature in serum and its significance for early diagnosis with chronic allograft nephropathy

作　　者：周绍棠[1] 阎冀焕[1] 杨成[1] 曾宁[1] 吴轲[1] 陈忠华 

机构地区：[1]华中科技大学同济医学院附属同济医院器官移植研究所,武汉430030

出　　处：《中华医学杂志》2006年第28期1966-1969,共4页National Medical Journal of China

基　　金：国家973高科技计划基金资助项目(2003CB5155)

摘　　要：目的用表面增强激光解吸离子化飞行时间质谱仪(SELDI-TOF-MS)检测慢性移植物肾病(CAN)患者血清蛋白质图谱,构建CAN血清蛋白质指纹图谱及其对该病的早期诊断作用。方法用SELDI-TOF-MS及弱阳离子交换芯片(CM10)检测24例移植肾功能完全正常的长期存活者(LS)和15例CAN患者的血清蛋白质图谱,并运用软件判别和分析处理数据,筛查特异的蛋白质分子标志物。结果对每份血清样本,质谱系统产生78个有意义的蛋白质峰,18个蛋白质的差异在两者间具有统计学意义(P<0．05),6个蛋白质(质荷比2476．0,3078．7,3190．5,4076．5,4506．0,6178．4)有极其显著差别(P<0．01),以质荷比3078．7为单一分子标志物可区别LS和CAN患者(P=0．0001),它在前者高表达,在后者低表达,其诊断CAN时的敏感性为87．5％,特异性为81．8％,其诊断作用绩效比为0．307。结论SELDI-TOF-MS构建的血清蛋白质指纹图谱在诊断CAN时具有较高的价值。Objective To construct protein fingerprints in serum and to early diagnose chronic allograft nephropathy （CAN） using surface enhanced laser ionization/desorption-time of flight-mass spectrometry （SELDI-TOF-MS）. Methods Serum protein spectra were detected by SELDI-TOF-MS and weak cation exchange （ CM10 ）, which were from long-term survival patients with well-functioning kidney allograft （KS） （n =24） and CAN （n = 15）, and then biomarkers were screened through the analysis of database with Biomarker Wizard and Biomarker Pattern softwares . Results 78 protein peaks of interest were generated in each sample, 18 protein spectra statistically show difference between KS and CAN （P 〈 0.05）, 6 of which （m/z 2476.0, 3078.7, 3190.5, 4076.5, 4506.0, 6178.4） statisticaUy show significant difference （ P 〈 0. 01 ）, a single biomarker （ m/z 3078. 7 ） differentiated LTS and CAN （ P = 0. 0001 ）, it was upregulated in the former and downregulated in the latter, its diagnostic sensitivity and specificity were 87.5% and 81.8% respectively. Its cost-effective value is 0.307. Conclusion Establishment of protein fingerprint signature in serum by SELDI-TOF-MS is clinically predictive of CAN.

关 键 词：肾移植 肾病 血清蛋白质 指纹图谱 

分 类 号：R699[医药卫生—泌尿科学]