坐骨神经再生过程中施万细胞自噬的形态学观察  

Morphological observation of Schwann cell autophagy during regenerative course of sciatic nerve

作　　者：王启伟[1] 迟德彪[2] 路艳蒙[3] 朴仲贤[4] 王万山[5] 顾为望[5] 朴英杰[2] 

机构地区：[1]中国军事医学科学院生物工程研究所,北京100071 [2]南方医科大学基础医学院,广州510515 [3]南方医科大学分析测试中心,广州510515 [4]汕头大学医学院中心实验室,广东汕头515041 [5]南方医科大学实验动物中心,广州510515

出　　处：《中国临床解剖学杂志》2006年第4期410-412,共3页Chinese Journal of Clinical Anatomy

基　　金：国家自然科学基金(30300191)

摘　　要：目的:探讨大鼠坐骨神经再生过程中的细胞自噬作用。方法:横切大鼠坐骨神经制作神经损伤再生模型,分别于造模后0、0.5、1.0、1.5、2.0、3.0、4、5、7、10、15d取近断端组织行电镜结构观察。结果:轴突在第1天时从髓鞘脱离,溃变呈空泡状。第2天开始髓鞘皱褶、绞窄并脱落形成碎片,施万细胞内可见大小形状各异的髓鞘碎片,并与大量溶酶体融合形成自噬泡,呈酸性磷酸酶(AcPase)阳性。第7d时幼稚细胞出现于新生毛细血管周围,大量幼稚细胞随后出现。结论:施万细胞自噬对坐骨神经再生时溃变髓鞘的清除起主要作用,溶酶体显著地参与了该过程,施万细胞脱分化为幼稚的祖细胞后大量增殖分化参与神经再生过程。Objective： To investigate the autophagic effects of Schwann cell during regenerative course of rat sciatic nerve. Methods： Nerve injury and regeneration model was made by transecting rat sciatic nerve. Nerve samples from the proximal end of injuring nerves were obtained 0, 0.5, 1, 1.5, 2, 3, 4, 5, 7, 10, 15 days after transection respectively and ultrathin sections were prepared for examination under electron microscope. Results： Axons started to separate from myelin sheath at the first day after transection and degenerated quickly with vacuole appearance. And then, myelin sheath creased, strangled and fell off into fragments, and large amounts of myelin debris of different sizes and shapes appeared in Schwann cells and fused with lysosomes to form AcPase reaction-positive autophagic vacuoles. Juvenile cells were seen firstly around the newly-born blood capillary at the 7th day after transection and many of them appeared later. Conclusion： The degenerating myelin debris is cleared mainly by autophagy of Schwann cells during regeneration of rat sciatic nerve and lysosomes play a remarkable role in the process. Schwann cells dedifferentiated into young cells named precursor Schwann cell, then proliferate and differentiate to take part in the regeneration of nerves.

关 键 词：施万细胞 自噬 神经再生 

分 类 号：R329.24[医药卫生—人体解剖和组织胚胎学]