Formation of microchimerism in rat small bowel transplantation by spienocyte infusion  

作　　者：Da-Xun Piao Tao Jiang Lian-Xin Liu An-Long Zhu Shao-Feng Jin Ying-Hui Guan 

机构地区：[1]Department of General Surgery, the First Affiliated Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, China [2]Department of General Surgery, the Affiliated Hospital of Harbin Institute of Technology, Harbin 150001, Heilongjiang Province, China [3]Department of General Surgery, the Fourth Affiliated Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, China

出　　处：《World Journal of Gastroenterology》2006年第26期4166-4169,共4页世界胃肠病学杂志（英文版）

基　　金：Supported by grant from Program for Innovative Ability of Key Teachers in Universities of Heilongjiang Province

摘　　要：AIM： To investigate the effect of donor splenocyte infusion combined with cyclosporine A （CsA） on rejection of rat small bowel transplantation （SBT）. METHODS： Male Sprague-Dawley （SD） rats and female Wistar rats weighing 230-270 g were used as donors and recipients respectively in the study. Heterotopic small bowel transplantation was performed. The rats were divided into three groups： group one receiving allotransplantation （SD→Wistar）, group two receiving allotransplantation （SD→Wistar） ＋ donor splenocyte infusion, group three receiving allotransplantation （SD →Wistar） ＋ donor splenocyte infusion ＋ CsA followed by CsA 10 mg/kg per day after transplantation, in which recipient Wistar rats were injected with 2 ×10^8 SD splenocytes 28 d before transplantation, and treated with CsA after transplantation. Finally, the specific DNA fragment of donor Y chromosome was detected in recipient peripheral blood and skin by PCR. The survival time after small bowel transplantation was observed. Gross and histopathological examinations were performed. RESULTS： The survival time after small bowel transplantation was 7.1 ± 1.2 d in group 1, 18.4 ± 3.6 d in group 2 and 31.5± 3.1 d in group 3. The survival time was significant longer （P 〈 0.01） in group 3 than in groups 1 and 2. The gross and histopathological examination showed that the rejection degree in group 3 was lower than that in groups 1 and 2.CONCLUSION： Donor splenocyte infusion combined with CsA decreases remarkably the rejection and prolongs the survival time after rat small bowel transplantation.瞄准：为了调查施主脾细胞的效果，注入在老鼠的拒绝上与 cyclosporine A (CsA ) 结合了小肠移植(SBT ) 。方法：男 Sprague-Dawley (SD ) 老鼠和女 Wistar 老鼠称 230-270 g 在学习分别地被用作施主和接受者。异位的小肠移植被执行。老鼠被划分成三个组：组织收到紧密相联的移植( SD rarr Wistar )的，组织二收到紧密相联的移植( SD rarr Wistar )+施主脾细胞注入，组织三收到+施主脾细胞注入+ CsA 在移植以后每天由 CsA 10 mg/kg 跟随了的紧密相联的移植( SD rarr Wistar )，在哪个接受者 Wistar 老鼠与 2 x 10 被注射( 8 ) SD splenocytes 在移植前的 28 d ，并且在移植以后与 CsA 对待。最后，施主 Y 的特定的 DNA 碎片被 PCR 在接受者外设血和皮肤检测。在小肠移植以后的生存时间被观察。粗野、组织病理学说的考试被执行。结果：在小肠移植以后的生存时间是 7.1 +/- 在组 1 的 1.2 d， 18.4 +/- 在组 2 和 31.5 +/- 的 3.6 d 在组 3 的 3.1 d。生存时间更长是重要的(P < 0.01 ) 在比在组的组 3 1 和 2。粗野、组织病理学说的考试证明在组 3 的拒绝度在组是比那低的 1 和 2。结论：与 CsA 相结合的施主脾细胞注入显著地减少拒绝并且在老鼠以后延长生存时间小肠移植。

关 键 词：MICROCHIMERISM Splenocyte infusion Immunologic tolerance Small bowel transplantation 

分 类 号：R656.7[医药卫生—外科学]