雌激素缺乏对大鼠腹主动脉瘤模型形成的影响  

Influence of estrogen deficiency in the formation and development of experimental abdominal aortic aneurysm

作　　者：吴晓飞[1] 张健[1] 辛世杰[1] 段志泉[1] 张弓 

机构地区：[1]中国医科大学附属第一医院普外三科,辽宁沈阳110001 [2]盘锦市第一人民医院妇科,辽宁盘锦124010

出　　处：《中华肿瘤防治杂志》2006年第12期888-891,共4页Chinese Journal of Cancer Prevention and Treatment

基　　金：国家自然科学基金(30471706)

摘　　要：目的:建立雌激素缺乏的动物模型并在此基础上建立腹主动脉瘤(abdominalaorticaneurysm,AAA)模型,研究雌激素缺乏对AAA形成中的影响及其作用机制。方法:雌性Wistar大鼠20只,随机分为实验组和对照组,各10只。实验组(切除卵巢)和对照组(假手术)分别于术后1周用弹性蛋白酶灌注肾下腹主动脉制成AAA模型。14d后用放射免疫法检测血清E2的含量,用免疫组化及RealTimeRTPCR测定动脉瘤组织基质金属蛋白酶(matrixmetalloproteinase,MMP)2和MMP9的mRNA及蛋白的表达。结果:实验组与对照组比较雌激素水平显著降低,P<0.01。其动脉瘤扩张率分别为(160.52±18.38)%和(101.30±6.73)%,P<0.01;MMP2mRNA的相对表达量分别为0.128±0.070和0.014±0.006,P<0.01;MMP9mRNA的相对表达量分别为1.50±0.43和0.33±0.11,P<0.01;MMP2的表达率分别为(48.51±9.63)%和(25.33±3.15)%,P<0.01;MMP9的表达率分别为(46.46±5.91)%和(24.78±2.30)%,P<0.01。结论:在AAA形成的过程中雌激素缺乏可导致MMP2和MMP9的mRNA表达增强,MMP2和MMP9合成及炎性细胞浸润的增加,进一步促进AAA的形成。OBJECTIVE： To investigate the mechanism of estrogen deficiency in the formation and development of experimental abdominal aortic aneurysm. METHODS： Female Wister rats were divided into experiment group and control group randomly. Rats in experiment group were ovariectomized and those of control group were sham-operated. Elastase perfusion of the infrarenal aorta was performed in rats. At 14 days, estrogen was detected by radioimmunoassay and aortas were harvested for immunohistochemistry and real-time RT-PCR. RESULTS： Experimental group showed a lower estrogen level than that of control group, P〈0.01. Aneurysms dilatation rates of experiment group and control group Were（160.52±18.38）% and （101.30±6.73）% respectively（P〈0.01）. Their relative expression quantities of MMP-2 mRNA were 0. 128±0.070 and 0.014±0.006 respectively（P〈0.01） and those of MMP-9 mRNA were 1. 50±0.43 and 0.33±0. 11 respectively（P〈0.01）. MMP-2 expression of experiment group and control group were （48. 51±9.63） and （25.33±3.15）% respectively （P〈0.01） and those of MMP-9 were （46.46±5.91）% and （24.78±2.30）% respectively （P〈0.01）. CONCLUSIONS： In AAA development, estrogen deficiency may promote the AAA formation by enhance MMP-2 and MMP-9＇s expression of mRNA and protein and inflammatory cell infiltrate.

关 键 词：主动脉瘤 腹/酶学 疾病模型 动物 灌注 局部 基质金属蛋白酶类/分析 免疫组织化学 逆转录聚合酶链反应 

分 类 号：R543[医药卫生—心血管疾病]