膜受体介导的细胞凋亡与细胞增殖  

Membrane receptor-mediated apoptosis and proliferation

作　　者：杨长永[1] 周毅[1] 龚建平[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院肿瘤研究所普外科,湖北武汉430030

出　　处：《中华肿瘤防治杂志》2006年第13期986-989,共4页Chinese Journal of Cancer Prevention and Treatment

基　　金：卫生部临床重点学科资助项目(20012537)

摘　　要：目的：观察膜受体介导的人类细胞凋亡与细胞增殖，阐明膜受体介导的细胞凋亡与细胞增殖的关系。方法：用肿瘤坏死因子-α、抗Fas抗体分别处理指数生长期的Molt-4、Jurkat以及健康人外周血淋巴细胞；应用SubGl法和AnnexinV／PI等方法通过流式细胞仪检测细胞增殖和细胞凋亡。结果：处于静止期的外周血淋巴细胞对凋亡诱导剂不敏感，凋亡率是6％-8％。Molt-4、Jurkat细胞以及加入PHA刺激的外周血淋巴细胞经肿瘤坏死因子一a、抗Fas抗体诱导后出现了明显的细胞凋亡，凋亡率是15％-28％。经肿瘤坏死因子-α、抗Fas抗体诱导后，进入细胞周期增殖的细胞较静止期的细胞凋亡率有明显增加。结论：膜受体介导的细胞凋亡与细胞增殖具有协同作用。OBJECTIVE： To investigate the membrane receptor-mediated apoptosis and cell proliferation specificity in cultured normal and tumour lymphocytes and the relationship between apoptosis and cell cycle. METHODS： Exponentially growing human leukaemia cell lines（Molt-4, Jurkat） and peripheral blood lymphocyte （PBL） were treated with TNF-α or Anti-Fas. PBL were stimulated by phytohemagglutinin （PHA） and further incubated with the presence of TNF-αor Anti-Fas. Method of Annexin V/PI was used to detect the apoptosis, and method of API was applied to illustrate the cell cycle specificity of apoptotic cells. RESULTS： Unstimulated PBL kept blunt to insults of TNF-α or Anti-Fas, and apoptotic rate was 6%-8%. Molt-4, Jurkat and stimulated PBL which were treated by TNF-α or Anti-Fas went to apoptosis, and the rate was 15%-28%. Most apoptosis happened in early Glphase. CONCLUSION： Receptor-mediated apoptosis is closely related to cell cycle and presents cell cycle specificity.

关 键 词：细胞增殖 细胞凋亡 外周血淋巴细胞 膜受体 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]