大鼠腰、颈段脊髓神经元间联系的c-fos基因研究  

A c-fos labeling study on the neuronal pathway connection between the cervical spinal cord and the lumbar spinal cord

作　　者：张敬东[1] 张新[2] 袁绍辉[2] 俞兴[3] 徐林[3] 

机构地区：[1]解放军第二零八医院骨科,长春130062 [2]吉林大学第一医院骨科 [3]北京中医药大学东直门医院骨科

出　　处：《中国康复医学杂志》2006年第7期588-589,i0001,共3页Chinese Journal of Rehabilitation Medicine

基　　金：吉林省科委资助课题(20030509)

摘　　要：目的应用c-fos基因研究腰段和颈段脊髓神经元之间的联系。方法成年雄性SD大鼠20只,钳夹左侧坐骨神经,灌注取材后行c-fos免疫组化染色。结果在腰段L4-6脊髓中,钳夹诱发的c-fos基因表达均在钳夹同侧脊髓灰质内,脊髓灰质前角神经元和后角神经元中的c-fos基因表达明显增多,对照侧右侧脊髓中的神经元c-fos基因未见明显表达。在颈段C5-8脊髓中,双侧脊髓灰质前角中可见c-fos基因表达明显增多,双侧脊髓灰质后角中未见明显c-fos基因表达。结论颈段脊髓和腰段脊髓神经元之间存在着神经纤维联系。Objective：To investigate the neuronal pathway connection between the cervical spinal cord and the lumbar spinal cord with c-fos labeling. Method： 20 male SD rats were operated to expose and grind the left sciatic nerve with forceps. The cervical and lumbar spinal cord was taken out 2 hours after the operation.Then the im- munochemical labeling of the c-fos was done. Result：The anterior corner cell and posterior corner cell with c-fos labeling were found in the left lumbar spinal cord （L4-6）,and it was found in the anterior corner of the two side cervical spinal cord（CS-8） too.Conclusion： There is neural pathway connection between the cervical spinal cord and the lumbar spinal cord.

关 键 词：坐骨神经 C-FOS基因 脊髓 神经元 

分 类 号：R346[医药卫生—基础医学]