高血压大鼠肥厚心肌和主动脉中PER 2蛋白表达的变化  

Expression of PER 2 protein in hypertrophic myocardium and aorta of hypertensive rats

作　　者：丁延魁[1] 黄建寨[1] 张莉[1] 钟晓华[1] 李庆平[1] 

机构地区：[1]南京医科大学心血管药理研究室

出　　处：《中国临床药理学与治疗学》2006年第6期625-628,共4页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：国家自然科学基金资助项目(№30271506)

摘　　要：目的:以Sprague-Dawley(SD)大鼠作对照,测定自发性高血压大鼠(SHR)心肌和主动脉中PER 2(period 2)蛋白的表达。方法:取SHR及正常SD大鼠,在每12 h光暗交替的环境中饲养2周后,每隔4h取心脏和胸主动脉,Western blot方法检测SHR与正常大鼠心肌和主动脉组织中PER 2蛋白的表达。结果:正常大鼠和SHR心肌和主动脉组织中PER 2蛋白表达都呈现出明显的节律性,但SHR大鼠心肌组织和主动脉组织中PER 2蛋白的表达水平高于正常大鼠。结论:高血压大鼠肥厚心肌和主动脉组织中PER 2蛋白表达与正常大鼠中的表达存在差异,高血压导致心肌肥厚和主动脉病变与时钟蛋白表达异常之间可能有着互相促进的关系。AIM： To explore the expression of PER 2 （Period 2） protein, one clock protein, in aorta and myocardiums from Sprague-Dawley（SD） rats and spontaneous hypertensive rats （SHR）. METHODS： Spontaneous hypertensive rats and SD rats were housed in a dark room for 36 h, followed by a strict 12 h light/12 h dark cycle regimen for 2 weeks （lights on at 09 ： 00, zeitgeber time [ZT] 0）. On the day of experiment, rats were sacriticed every 4 h. The expression of PER 2 protein was detected by Western blot analysis. RESULTS： PER 2 protein expression exhibited a rhythm of approximately 24 h in normal myocardium and aorta, and its expression in hypertrophic myocardium and aorta also exhibited this rhythm. However, PER 2 protein expression of SHR was significantly higher than that of normal rats. CONCLUSION： There might be a positive relationship between hypertension-induced myocardial hypertrophy and aortal disorder and the enhancement of expression of clock protein.

关 键 词：时钟基因 PER 2蛋白 高血压 心肌 主动脉 

分 类 号：R965.1[医药卫生—药理学] R544.1[医药卫生—药学]