奶牛日粮添加豆油抑制乳腺硬脂酰辅酶A去饱和酶(SCD)基因mRNA表达水平  

Addition of Soybean Oil to Diets for Dairy Cows Decrease SCD Gene Expression in Mammary Gland

作　　者：卜登攀[1] 王加启[1] 刘仕军[1] 刘晓云[1] 谷俊[1] 张瑞超[1] 

机构地区：[1]中国农业科学院北京畜牧兽医研究所动物营养学国家重点实验室,北京100094

出　　处：《中国畜牧兽医》2006年第7期3-5,共3页China Animal Husbandry & Veterinary Medicine

基　　金：科技部国家"十五"科技攻关计划"奶业重大关键技术研究与产业化技术集成示范"(2002BA51802)

摘　　要：硬脂酰辅酶A去饱和酶(SCD)是奶牛乳腺合成共轭亚油酸(CLA)的关键酶。奶牛日粮饲喂35 d豆油后,与不添加豆油的对照组相比,抑制了奶牛乳腺组织SCD基因mRNA的表达,研究结果表明,日粮亚油酸对乳腺SCD酶基因表达具有抑制作用。The experiment was conducted to determine the effect of dietary soybean oil on Stearoyl-CoA-desaturase （SCD） gene expression in mammary gland. Multiparous Hostein cows （n= 6）in middle lactation were used in a completely randomized design. Cows were given a total mixed ration with no added soybean oil （CTL） or soybean oil （TRT, about 2% of dry matter） for 5 weeks. Mammary gland tissue was taken from a rear quarter at the end of the treatment period. Consumption of soybean oil decreased the abundance of SCD mRNA in the mammary gland by 50% compared to control group. The results suggest that soybean oil decrease expression of SCD gene mainly due to the intake of linoleic acid.

关 键 词：豆油 硬脂酰辅酶A去饱和酶(SCD) 基因表达 实时定量PCR(RT—PCR) 奶牛 

分 类 号：S823.91[农业科学—畜牧学]