显微动态图像分析技术及其在生物物理中的应用  

An analytical technology of dynamic micrograph and application in biophysics

作　　者：阮萍[1] 雍军光 黄耀熊[3] 林绍和[1] 

机构地区：[1]桂林医学院物理教研室,广西桂林541004 [2]桂林市第二人民医院内分泌科,广西桂林541001 [3]暨南大学生物医学工程研究所,广州510632

出　　处：《光学技术》2006年第4期490-492,495,共4页Optical Technique

基　　金：国家自然科学基金资助项目(30227001);广西教育厅科研资助项目(2001035)

摘　　要：介绍了一种可实时监测细胞生命活动的显微动态图像分析技术。该技术把显微光学成像技术、数字处理技术和动态图像分析技术结合为一体,可实现对活细胞的结构功能、生长过程和环境变化的影响等进行非侵入性连续监测。通过在细胞膜弹性模量测量中的应用表明,该技术不仅原理可靠,实现容易,应用方便,而且检测速度快,测量精度高,稳定性能好。此项技术可望在生物物理学的实验研究中获得广泛的应用。An analytical technology that may be used to dynamically monitor real-time micrographs is introduced in live activities of cells. Integrating microphotographic imaging technology with digital signal processing and dynamic image analysis technologies, this analytical technology might be used to do not invasive monitoring on cellular architecture and function, growth procedure and influences of environmental changes. The result of applications in measuring elastic modulus of cellular membranes indicates that the technology is reliable in principle, easy for implementation and convenient in applications. In addition, this technology has more advantages such as faster detection speed, well measurement accuracy and higher reliability. It may widely be used in the research and experiments of biophysics.

关 键 词：显微动态图像分析 活细胞 弹性模量 

分 类 号：Q27[生物学—细胞生物学]