基于氧荧光猝灭速率法的生化需氧量检测  

BOD Measurement Based on Oxygen Fluorescent Quenching Rate Method

作　　者：肖来龙[1] 辛玲玲[1] 赵丽[1] 章丽燕[1] 陈曦[1] 王小如[1] 

机构地区：[1]厦门大学化学化工学院化学系现代分析科学教育部重点实验室

出　　处：《化学通报》2006年第7期508-513,共6页Chemistry

基　　金：国家863计划(2001AA635100);福建省自然科学基金(D0410001)资助项目

摘　　要：利用氧荧光碎灭速率的方法，结合自行构建的BOD光纤传感装置进行海水中生化需氧量（BOD）含量检测。考察了四种筛选的海洋耗氧菌种在四甲基硅氧烷（TMOS）、二甲基二甲氧基硅烷（DiMeDMOS）和聚乙烯醇（PVA）包埋固定情况下，对不同浓度的葡萄糖-谷氨酸（GGA）标准溶液的荧光响应情况。BOD敏感膜的荧光响应在0．2～30mg／L浓度范围内呈良好的线性关系，对2mg／L标准溶液测定的相对标准偏差为2．5％（n=6），响应时间（t95%）为4．0min，BOD敏感膜使用寿命大于12个月。实际海水样品检测表明，利用BOD敏感膜检测得到的结果与国标BOD，方法之间存在较好的一致性。Based on oxygen fluorescent quenching rate method,a BOD optical sensing film immobilized four kinds of microorganisms from seawater in an Ormosils-PVA matrix was applied for BOD measurement of seawater. The responding characteristic of fluorescent quenching is researched. The BOD sensing film contributed a good linear relationship between concentration and fluorescent response in the concentration range of 0.2 to 30mg/L. Measurements were taken for 4min followed by 10min recovery, time in 2mg/L glucose/glutamate （GGA） BOD standard solution. Some other experimental conditions, such as pH. measurement temperature, the concentration of sodium chloride, were studied. BOD values estimated by this optical BOD sensing film correlate well with those determined by the conventional BOD5 method for seawater samples.

关 键 词：荧光碎灭速率 生化需氧量(BOD) 光纤传感器 

分 类 号：X832[环境科学与工程—环境工程]