禽肉中克球酚气相色谱微量快速测定法  

Rapid Determination of Clopidol Residue in Poultry Meat by Gas Chromatography

作　　者：牟峻[1] 荣会[1] 陈明岩[1] 李庆才[1] 

机构地区：[1]吉林进出口商品检验局

出　　处：《色谱》1996年第6期478-480,共3页Chinese Journal of Chromatography

摘　　要：对禽肉样品中克球酚药物残留采用微量化学法技术，以甲醇提取，氧化铝、阴离子树脂交换柱净化，乙酰化后，进行ＧＣ－ＥＣＤ毛细管色谱测定。经对肉鸡、填鸭、饲养山鸡进行添加回收率试验，方法回收率为８５．７％～９４．４％，变异系数为３．８％～７．７％，最低检出限为２．０ｎｇ／ｇ。This paper describes a rapid method for determination of clopidol in poultry meat. The clopidol is extracted from the meat tissues with methanol, and cleaned up on a micro column packed with PT neutral alumina and one packed with 100 200 anion exchange resin to remove the impurities and fat. Then clopidol is acetylated with acetic anhydride, forming clopidol acetate, which is determined by capillary gas chromatography with electron capture detector by using an HP 50+ 30m×0.53mm×1.0μm column, The presence of clopidol acetate is further confirmed by using an HP 5 25m×0.32mm×0.25μm column. External standard method is used for quantitation. The average recovery and CV ranges were 85.7% 94.4% and 3.8% 7.7% respectively. The detection limit was 2.0ng/g.

关 键 词：气相色谱法 克球酚 禽肉 向量化学法 

分 类 号：S859.795[农业科学—临床兽医学]