高效细内径毛细管电色谱填充柱的制备  

Preparation of High Efficent Capillary Packed Columns with Narrow Internal Diameter for Electrochromatography

作　　者：施维[1] 邹汉法[1] 张津[1] 董礼孚[1] 张玉奎[1] 

机构地区：[1]中国科学院大连化学物理研究所,国家色谱研究分析中心

出　　处：《色谱》1996年第5期351-353,共3页Chinese Journal of Chromatography

基　　金：中科院院长基金

摘　　要：发展了一套毛细管填充柱制备方法，在１００μｍ内径毛细管柱中填充３μｍＯＤＳ固定相，以毛细管电色谱法（ＣＥＣ）分离模式对芳香胺类样品进行分离，柱效高达２５．９万理论塔板数／米，折合塔板高度达１．３。类似研究在国内尚未见报道。The packed-column capillary. electrochromatography (CEC) method combine the advantages of CE with those of HPLC, and can provide high efficient separation and good selectivity of complex samples. In this paper, a new method was developed to prepare packed column for capillary electrochromatography. The fused silica capillary column with 100μm inner diameter was packed with 3μm ODS HPLC stationary phase to 60cm within 10 min. The electroosmotic mobility was 5. 76mm/(s. V) measured by thiourea. The baseline separation of aromatic alcohols and aromatic amines (k'= 0-4. 2) were performed on a home-made CE apparatus. A column efficiency of 2. 59 × 105 theoretical plates/m was achieved, and the reduced plate height was 1. 3. The column can be regenerated by cutting off the injection end. From the results, it can be seen that, by optimizing the separation conditions, the CEC method can be applied efficiently to the field of the environmental and medicine analysis.

关 键 词：毛细管电色谱泮 毛细管填充柱 芳香醇 芳香胺 

分 类 号：O657.8[理学—分析化学]