高效液相色谱法测定CHP与有关物质含量  

Determination of CHP and related substances by HPLC

作　　者：郭平[1] 刘玉真[1] 聂新永[1] 张劲影[1] 

机构地区：[1]河北科技大学化学与制药工程学院,河北石家庄050018

出　　处：《河北工业科技》2006年第4期222-223,227,共3页Hebei Journal of Industrial Science and Technology

摘　　要：采用Shim-pack CLC-SIL(4.6 mm×250 mm,5μm)色谱柱,建立了高效液相色谱法以乙腈-Ⅰ为流动相进行分离(其中Ⅰ为配制的稀硫酸溶液),二极管阵列检测器在230 nm波长处检测,测定CHP含量与有关物质。进样体积为10μL,流速为1 mL.min-1。High performances liquid chromatography method for analyzing CHP and related substance was established by Shimpack CLC-SIL （4.6 mm×250 mm,5 μm）column. Gradient was adopted at the detection of 230 nm with a diode array detector. The quanitity of paeoniflorin was 10 μL. The flow rate was 1 mL · min^-1.

关 键 词：高效液相色谱法 CHP 有关物质 

分 类 号：O657.72[理学—分析化学]