反相色谱法分离和间接光度法测定无机阴离子  

Determination of Inorganic Anions by Liquid Chromatography and Indirect Photometric Detection

作　　者：张孝松[1] 储著银[1] 徐霁昀[1] 林明[1] 林长山[1] 

机构地区：[1]中国科学技术大学应用化学系

出　　处：《色谱》1996年第5期397-399,共3页Chinese Journal of Chromatography

摘　　要：在化学键合固定相ＯＤＳ柱上，用Ｆｅ（Ｐｈｅｎ）_３^（２＋）作流动相添加剂分离并用间接光度法检测无机阴离子。探讨了主要因素（如流动相中Ｆｅ（Ｐｈｅｎ）_３^（２＋）浓度、有机溶剂浓度、ｐＨ值和离子强度等）对阴离子保留和检测灵敏度的影响。Ｃｌ~－，Ｂｒ~－，ＮＯ_３~－和Ｉ~－具有良好的分离度；间接光度检测波长为Ｆｅ（Ｐｈｅｎ）_３^（２＋）的最大吸收波长５１０ｎｍ。测定这４个离子的校正曲线的线性范围为１～１６０ｍｇ／Ｌ（Ｃｌ~－，Ｂｒ~－，ＮＯ_３~－）和２～３２０ｍｇ／Ｌ（Ｉ~－）；相关系数均达０．９９６。检测限（以Ｓ＝２Ｎ计）分别为１．０ｎｇ（Ｃｌ~－），１．７ｎｇ（Ｂｒ~－），１．９ｎｇ（ＮＯ_３~－）和５．６ｎｇ（Ｉ~－）。方法应用于雨水和自来水中Ｃｌ~－的测定，并获得满意的结果。Fe(Phen)3SO4 was used as a mobile phase additive for the liquid chromatographic separation of inorganic analyte anions on a Zorbax ODS column (250mm×4. 6mm i. d. ). The effects of organic modifier, concentration of Fe(Phen)3SO4, pH value and ionic strength in the mobile phase on the retention times and detection sensitivities of analyte anions had been investigated. The excellent resolution for CI, Br, NO3 and I using the mobile phase containing 1.0 ×1 0-4mol/L Fe (Phen)3SO4 at a flow rate of 1.0mL/min. Indirect photometric detection was used to detect the analyte anions by monitoring the eluate at 510nm where Fe (Phen) exhibits absorption maximum. The calibration graphs were linear over 1-160mg/L for Cl, Br and NO3, and 2-320mg/L for I. The detection limits were 1.0, 1.7, 1.9 and 5.6ng for CI, Br, NO, and I respectively.The proposed method has been applied to analyze rain and tap water and satisfactory results have been obtained.

关 键 词：反相高效液相色谱法 无机阴离子 间接光度检测 

分 类 号：O652.63[理学—分析化学]