HPLC分离化学发光检测药物中的马钱子碱、士的宁和麻黄类生物碱  

Chemiluminescence Determination of Brucine,Strychnine and Ephedrines Based on HPLC Separation

作　　者：朱龙[1] 封满良[1] 万秀琴[1] 吕九如[1] 

机构地区：[1]陕西师范大学化学系

出　　处：《高等学校化学学报》1996年第11期1693-1696,共4页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金

摘　　要：在ＫＭｎＯ４－Ｈ＋－还原剂体系中研究了马钱子碱、士的宁和麻黄类生物碱的化学发光（ＣＬ）行为，建立了其快速灵敏的流动注射化学发光分析方法，将此法与ＨＰＬＣ联用，实现了上述物质的选择性测定，该方法的检出限为１．０×１０－１０ｇ／ｍＬ马钱子碱、１．０×１０－８ｇ／ｍＬ士的宁、１．０×１０－５ｇ／ｍＬ麻黄碱和伪麻黄碱，测定的相对标准偏差小于３％，ＨＰＬＣ流动相的脉动及其中的溶解氧不影响测定的精密度。此法已用于一些药物中马钱子碱等生物碱的测定，回收率为９４％～１０１％。In this paper,the chemiluminescence reaction of brucine,strychnine and ephedrines in KMnO4-H+-reductant system has been characterized with respect to their ability to act as a rapid and sensitive chemiluminescence determination system of the above alkaloids.This method has been connected with HPLC which fitted with a C18 column for separation so as to determine these alkaloids in sample.The detection limits are 1.0×10-10g/mLof brucine,1.0×10-8g/mL of strychnine and 1.0×10-5 g/mL of ephedrines, respectively.The relative standard deviation is less than 3%and recovery is 94%～101%.Since the dissolved oxygen did not affect the determination,this method provide advantages of wider linear range,high sensitivity and easy to operate.

关 键 词：HPLC 化学发光分析 高锰酸钾 生物碱 中药化学 

分 类 号：R284.1[医药卫生—中药学]