乙型肝炎疫苗-卡介苗联合疫苗在动物中的免疫应答  

Immune Response Induced by HB-BCG Combined Vaccine in Animals

作　　者：杨丽华[1] 王志[1] 赵小琳[1] 杨彤[1] 于爱东[1] 刘艾红[1] 金立杰[1] 

机构地区：[1]长春生物制品研究所疫苗五室,长春130062

出　　处：《中国生物制品学杂志》2006年第4期378-380,共3页Chinese Journal of Biologicals

基　　金：吉林省科技发展计划项目(20050402-1).

摘　　要：目的评价乙型肝炎疫苗-卡介苗联合疫苗的免疫应答。方法用联合疫苗及其单价疫苗分别免疫豚鼠和BALB/c小鼠,检测两种疫苗的免疫应答。结果联合疫苗与卡介苗豚鼠皮肤PPD反应直径数值相近(P>0·05),联合疫苗与乙肝疫苗免疫小鼠血清乙肝抗体滴度差异无显著意义(P>0·05);联合疫苗免疫小鼠淋巴细胞转化刺激指数、IL-2含量均高于单价疫苗,差异有显著意义(P<0·001)。结论乙型肝炎疫苗-卡介苗联合疫苗能有效地诱导实验动物的体液免疫和细胞免疫。Objective To evaluate the immune response induced by HB-BCG combined vaccine. Methods Immunize guinea pigs and BALB/c mice with hepatitis B（HB） vaccine,BCG vaccine and HB-BCG combined vaccine separately and determine the immune responses induced in the animals. Results The skin reaction diameters in PPD test of guinea pigs immunized with HB-BCG combined vaccine showed no significant difference from with BCG vaccine（P 〉 0. 05 ）. No significant difference was observed between the serum HB antibody titers of mice immunized with HB-BCG combined vaccine and HB vaccine（ P 〉 0. 05 ）. The lymphocyte transformation stimulating index and IL-2 level induced by HB-BCG combined vaccine were significantly higher than those induced by monovalent HB or BCG vaccine（ P 〈 0. 001 ）. Conclusion HB-BCG combined vaccine effectively induced beth humoral and cellular immune responses in animals.

关 键 词：乙型肝炎疫苗 卡介苗 联合疫苗 免疫应答 

分 类 号：R512.620.3[医药卫生—内科学] R186[医药卫生—临床医学]