川芎嗪液对大鼠视网膜缺血再灌注损伤模型的影响  

Effects of the Ligustrazine Injection on Retina Injury Rat Models Induced by Retina Ischemia-reperfusion

作　　者：练美莲[1] 孙砚辉[1] 陈苑[2] 王萧[2] 

机构地区：[1]广州中医药大学基础医学院,广州510405 [2]广州中医药大学动物实验中心,广州510405

出　　处：《中药新药与临床药理》2006年第4期260-261,285,共3页Traditional Chinese Drug Research and Clinical Pharmacology

摘　　要：目的观察中药川芎嗪液对实验性高眼压致视网膜缺血再灌注损害模型的治疗作用。方法32只SD大鼠随机分为4组:川芎嗪组、维生素E组、模型组和正常组。采用短暂升高大鼠前房压至110mmHg制作视网膜缺血再灌注模型。川芎嗪组采用川芎嗪液,维生素E组用维生素E作灌胃治疗,疗程7d。模型组和正常组灌胃等量蒸馏水。光镜、电镜观察结构的改变,并测定SOD、MDA含量变化。结果缺血60min再灌注48h以上,光镜下可见视网膜视神经节细胞层(RGCL)和内核层(INL)细胞明显变薄;电镜下可见RGCL、INL层环核状核固缩及凋亡小体出现;SOD活力下降、MDA含量上升。经川芎嗪液治疗后,RGCL、INL两层形态学有明显改善,SOD活力也有所提高,MDA含量下降。证明川芎嗪液具有一定抗缺血、抗缺氧及稳定细胞膜的功效。结论川芎嗪液对治疗视网膜缺血性疾病有疗效。Objective To investigate the therapeutic effect of the ligustrazine injection on rats models of experimental high intraocular pressure induced by retina ischemia - reperfusion. Methods Thirty two SD rats were divided into four groups randomly： the ligustrazine group, the model group, the normal group and the vitamine E group. The ischemia - reperfusion model is established by increasing intraocular pressure to 110mmHg in rat eyes. The ligustrazine group was treated with the ligustrazine injection, the vitamine E group with gastric gavage of vitamine E, and the model and normal groups with distilled water. The period of treatment was 7 days. The histopathologic changes were observed under the optical microscope and the electron microscope, meanwhile the SOD and MDA were measured. Results After ischemia for 60 min and reperfusion for 48 h, the cells in retinal ganglion cell layer and inner nuclear layer were thinned under optical microscope, perinuclear karyopyknosis and apoptotic bodies appeared in retinal ganglion cell layer and inner nuclear layer under electron microscope, SOD activity decreased and MDA level increased. After treatment, the morphology of retinal ganglion cell layer and inner nuclear layer was improved obviously, SOD activity increased and MDA level decreased. It is suggested that ligustrazine injection exerts anti - ischemic and anti - hypoxic and cytomembrane stabilizing effects to a certain extent. Conclusion The ligustrazine injection is an effective traditional Chinese medicine in treating retinal ischemia.

关 键 词：川芎嗪液 视网膜 缺血 形态学 自由基 SOD MDA 

分 类 号：R285.5[医药卫生—中药学]