大剂量肌苷对新生鼠缺氧缺血性脑损伤的治疗作用研究  

Study on the therapeutic effect of high dose inosine to hypoxi-schemic brain damage in neonatal rats.

作　　者：栾红[1] 高彬昌[1] 李琴 

机构地区：[1]青岛大学附属海慈医院儿科,青岛266033 [2]山东荣成市第二人民医院神经内科

出　　处：《中华妇幼临床医学杂志（电子版）》2006年第4期206-209,共4页Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition)

摘　　要：目的通过建立新生鼠缺氧缺血性脑损伤(hypoxic-ischemicbraindamage,HIBD)模型,探讨肌苷对新生鼠HIBD的治疗效果。方法将54只日龄为1周的新生鼠随机分为假手术组、对照组和肌苷组。造成新生鼠急性HIBD模型。肌苷组大鼠造模后立刻给予0.1%肌苷注射液(0.05mg/g),每日2次。假手术组和对照组给予相同剂量生理盐水。定时取标本,测定脑组织中水、钙含量及脑细胞凋亡率。结果1随着时间延长,脑组织水含量逐渐增加,5d达高峰,然后下降。与假手术组比较,对照组于3d和5d、肌苷组于5d皆有显著增高(P<0.05);与同时间点对照组相比,肌苷脑组织水含量于3d和5d皆有明显降低(P<0.05)。2脑组织中钙的含量24h最高,随着时间延长,逐渐下降,与假手术组比较,对照组于24h,3d和5d,肌苷组于24h和3d皆有显著增高(P<0.05);与同时间点对照组相比,肌苷组脑组织钙含量于24h,3d和5d皆有明显降低(P<0.05)。3随着时间延长,对照组和肌苷组脑细胞凋亡率逐渐增加(P<0.05);与假手术组相比,对照组和肌苷组各时间点的凋亡率皆有明显增高(P<0.05);与同时间点对照组相比,肌苷组的凋亡率明显降低(P<0.05)。结论肌苷能显著减轻由缺氧缺血所导致的神经细胞损伤和破坏,保护脑组织。Objective To explore the therapeutic effect of high dose inosine to hypoxic-ischemic brain damage （HIBD） in neonatal rats; and provide some evidences for wide clinical usage. Methods 54 neonatal rats aged 1 week were randomly divided into sham, control and inosine group. HIBD models were made. The rats in the inosine group began injecting 0. 1% inosine （0. 05 mg/g）, twice a day; and the rats in the sham and control group saline solution with the same dose. The samples were made at the given time; and the levels of water, calcium and cell apoptosis in brain were investigated. Results ①The levels of wate at 3 d, 5 d in the control group, and at 5 d in the inosine group were higher than that in the sham group （P〈0.05）. As compared with the control group at the given time, the levels in the inosine group at 3 d and 5 d were all significantly decreased （P〈0.05）.② The contents of calcium in brain tissues peaked at 24 h, and then gradually decreased. The contents at 24 h, 3 d, 5 d in the control group and 24 h, 3 d in the inosine group were all significantly increased in comparison with the sham group （P^0. 05）. As compared with the control group at the given time, the contents at 24 h, 3 d, 5 d in the inosine group were all significantly decreased（P〈0.05）. ③ The rates of brain cell apoptosis in the control and inosine group were all gradually increased （P〈0. 05）. The rates in the control and inosine group at every given time were all significantly increased （P〈0. 05）. The rates in the inosine group were all lower than that in the control group （P〈0.05）. Conclusion Inosine could remarkably protect cerebral tissue against HIBD.

关 键 词：肌苷 新生鼠 缺氧缺血性脑损伤 细胞凋亡 

分 类 号：R722.1[医药卫生—儿科]