锌和硒对二氧化硅致CHL细胞通讯连接的影响  

Effect of Zinc gluconate and Sidium selenite on gap junctional intercellual communication in fibroblast cells treated by silica

作　　者：谈伟君[1] 季晖[1] 焦石[2] 周敏[2] 

机构地区：[1]中国药科大学药学院药理教研室,南京210009 [2]东南大学公共卫生学院

出　　处：《中国公共卫生》2006年第8期966-968,共3页Chinese Journal of Public Health

摘　　要：目的观察葡萄糖酸锌、亚硒酸钠对二氧化硅致成纤维细胞通讯连接(GJIC)的单独及联合作用,寻找两者的最佳剂量组合。方法成纤维细胞与二氧化硅,同时加入不同浓度的葡萄糖酸锌、亚硒酸钠以及葡萄糖酸锌+亚硒酸钠溶液,共同培养6 h后借助划痕染料示踪技术观察成纤维细胞通讯连接功能。结果与二氧化硅组比,葡萄糖酸锌、亚硒酸钠可以显著减轻二氧化硅抑制成纤维细胞通讯连接功能作用(P<0.05);1.5 mg/L的葡萄糖酸锌与2.0μmol/L的亚硒酸钠联合作用效果最好。结论葡萄糖酸锌、亚硒酸钠单独和联合作用在体外可有效减轻二氧化硅粉尘致成纤维细胞通讯连接功能的抑制作用。Objective To investigate the effect of Zinc gluconate and Sodium selenite which were used solely and together on gap junctional intercellual communication（ GJIC） in fibroblast cells clited by silica. Methods Fibroblast cells were cultured with silica, at the same time Zinc gluconate and sodium selenite-which were used solely and together were added respectively. GJIC were measured through scrape loading and dye transfer. Results Zinc gluconate, sodium selenite and Zinc gluconate together with sodium selenite have the effects to lighten the level of GJIC in fibroblast coils inhibited by silica. The most suitable combiation of the two compound should be middle dosage of Zinc gluconate（ 1.5 mg/L）with high dosage of sodium selenite（2.0 μmol/L）. Conclusion Zinc gluconate and sodium selenite which were used solely and together could reduce the inhibition of GJIC in fibroblast cells exposed by silica.

关 键 词：二氧化硅 葡萄糖酸锌 亚硒酸钠 细胞通讯连接(GJIC) 

分 类 号：R114[医药卫生—卫生毒理学]