小孢子培养技术纯化与选育Pol TCMS不育两用系  

Development of Pol TCMS in Brassica napus through isolated microspore culture

作　　者：杨立勇[1] 刘平武[1] 杨光圣[1] 

机构地区：[1]华中农业大学作物遗传改良国家重点实验室国家油菜改良武汉分中心,湖北武汉430070

出　　处：《中国油料作物学报》2006年第2期115-118,124,共5页Chinese Journal of Oil Crop Sciences

基　　金：国家"973"计划(2001CB108807);国家"863"计划(2002AA207009);农业部农业结构调整重大专项(2002-06-02A)

摘　　要：利用甘蓝型油菜游离小孢子培养技术，获得了4个甘蓝型Pol细胞质温敏雄性不育两用系（Pol TCMS）的DH系，说明通过小孢子培养技术纯化选育Pol TCMS不育两用系是可行的。研究结果表明：Pol TCMS不育两用系的最佳取样时期为第一朵花开放前的4—7d；在NLN培养基中补充0．05mg／L的细胞分裂素6-BA可以显著提高出胚频率；游离小孢子首先在35℃高温条件下热激48h，然后转入到22℃恒温箱中连续暗培养效果最佳，其出胚频率显著高于其他3种温度组合；胚状体在8—10％条件下低温诱导两星期，能够显著提高其再生频率。The effects of inflorescence stage, N^6 - benzyladnine （6 - BA） concentration, temperature on embryo production from microspores of four Pol thermo -sensitive cytoplasmic male sterile （Pol TCMS） oilseed rape lines were investigated. The highest embryogenesis frequency was obtained from buds sampled at 4 - 7d before flowering and decreased with the aging of the inflorescence. High androgenic frequency was observed when the microspores were cultured in NLN - 13 solution medium containing 6 - BA of 0.05mg/L in the dark for 4 weeks. When the cultures were held at a high temperature of 35℃ for 48 h ,then maintained at a constgntly low temperature of 22℃, the highest number of the microspores from Pol TCMS lines ＂developed into embryos. The rate of planflet regeneration significantly increased after an initial period of cold induction （8 - 10℃ ） for two weeks.

关 键 词：甘蓝型油菜 Pol细胞质温敏雄性不育两用系 小孢子培养 DH系 

分 类 号：S565.403[农业科学—作物学]