四川彝族和新疆维吾尔族HLA-B基因座基因多态性分析  

Analysis of HLA-B Locus Gene Polymorphism in Sichuan Yi Ethnic Group and Xinjiang Uygur Ethnic Group

作　　者：许铭炎[1] 洪坤学[1] 马军[2] 邓小玲[3] 李军[3] 彭虹[1] 阮玉华[1] 秦光明[4] 张远志[2] 邢辉[1] 徐小虎[3] 邵一鸣 

机构地区：[1]中国疾病预防控制中心性病艾滋病预防控制中心,北京100050 [2]新疆维吾尔自治区疾病预防控制中心,乌鲁木齐830011 [3]汕头大学医学院病原生物学教研室,汕头515041 [4]四川省疾病预防控制中心,成都610031

出　　处：《遗传》2006年第8期913-917,共5页Hereditas（Beijing)

基　　金：国家杰出青年基金(编号:39925030);国家十五攻关项目(编号:2001BA705B02和2004BA719A01)资助~~

摘　　要：应用PCR-SSP方法对无亲缘关系的106位四川彝族样品和110位新疆维吾尔族样品进行HLA-B基因分型。在彝族样品中共检出21个等位基因,其中高频率的等位基因为B*40(0.1981)、B*15(0.1368)、B*51(0.1274),低频率的等位基因为B*47(0.0189)、B*44(0.0142)、B*18(0.0094)、B*57(0.0047)和B*78(0.0047),总体基因频率分布介于南北汉族之间。在维吾尔族样品中共检出27个等位基因,其中高频率的等位基因为B*35(0.1136)和B*51(0.1136),低频率的等位基因为B*41(0.0045)、B*56(0.0045)和B*78(0.0091),B*08、B*35、B*50等“高加索人”起源的HLA基因在新疆维吾尔族的分布频率高于国内其他民族,与高加索人种的数值相当。经χ2检验,两个民族群体的基因型分布均符合Hardy-Weinberg平衡。经遗传分析,四川彝族群体HLA-B基因座杂合度(H)、个体识别率(DP)和非父排除率(EP)分别为0.8977、0.9661和0.8009;维吾尔族群体的H、DP和EP分别为0.9372、0.9857和0.8732。获得了四川彝族和新疆维吾尔族HLA-B基因座等位基因频率数据,为临床器官移植配型、人类学、法医学及疾病关联性研究提供了重要的群体遗传学资料。The polymorphism of HLA-B alleles in Sichuan Yi and Xinjiang Uygur population was investigated using the PCR-SSP method. Twenty one alleles were detected in HLA-B loci in 106 Sichuan unrelated Yi healthy subjects. Of them, B ＊ 40, B ＊ 15 and B ＊ 51 were the most common alleles with an allele frequency of 0. 1981, 0. 1368, 0. 1274,respectively; while B ＊ 47, B ＊ 44, B ＊ 18, B ＊ 57and B ＊ 78 were the rare alleles with an allele frequency of 0. 0189, 0. 0142, 0. 0094, 0. 0047 and 0. 0047, respectively. The distribution of HLA-B allele frequencies in Sichuan Yis was between Southern Han and Northern Han. In 110 Xinjiang unrelated healthy Uygur subjects, 27 alleles were detected in HLA-B loci. Of them, B ＊ 35 and B ＊ 51 were the most common alleles with an allele frequency of 0.1136 and 0. 1136, respectively; while B ＊ 41, B ＊ 56 and B ＊ 78 were the rare alleles with a frequency of 0. 0045, 0. 0045 and 0. 0091, respectively. Frequencies of ＂Caucasoid origin＂ HLA alleles such＇ as B ＊ 08, B ＊ 35 and B ＊ 50 in Xinjiang Uygurs were higher than other ethnic groups in China. The result of x^2 tests showed that the distributions of HLA-B alleles in Yi and Uygur ethnic groups were in Hardy-Weinberg equilibrium. Heterozygosity （H）, discrimination power （DP） and probability of paternity exclusion （EP） of HLA-B locus from Sichuan Yi ethnic group were computed to be 0. 8977, 0. 9661 and 0. 8009 ; and those from Xinjiang Uygur ethnic group were 0. 9372, 0. 9857 and 0. 8732. The data obtained in this study on the distributions of HLA-B alleles in the Sichuan Yi and Xinjiang Uygur population provide important group genetics information for forensic and paternity tests to estimate the frequency of a DNA profile in these two populations, and can be Used in transplant matching, anthropological and disease association studies.

关 键 词：四川彝族 新疆维吾尔族 HLA-B 基因多态性 PCR-SSP 

分 类 号：Q987[生物学—遗传学]