离子交换-脉冲积分安培法分离检测氨基糖苷类抗生素  

Separation of several aminoglycoside antibiotics with ion-exchange column following with pulsed integrated amperometric detection

作　　者：蔡玉娥[1] 蔡亚岐[1] 牟世芬[1] 鲁毅强[2] 

机构地区：[1]中国科学院生态环境研究中心环境化学与生态毒理学国家重点实验室,北京100085 [2]北京科技大学化学系,北京100083

出　　处：《分析试验室》2006年第8期7-9,共3页Chinese Journal of Analysis Laboratory

基　　金：国家重点基础研究计划(973项目)(2003CB415001);国家自然科学基金(20475060;20577058);中国科学院方向性项目(KZCX3-SW-432);中国科学院优秀博士论文研究基金资助课题

摘　　要：用离子交换柱分离氨基糖苷类抗生素妥布霉素、新霉素B和西梭霉素，用脉冲积分安培电化学检测器进行检测。其流动相不同于一般抗生素的离子对色谱法，它配制简单，仅需使用NaOH-水体系；对两种检测电位波形进行了对比，并选取了表现优良的检测电位。方法对妥布霉素、新霉素B和西梭霉素的检出限分别为8、5、47ng／mL，在0．1～100／μg／mL范围内表现良好线性关系（r=0．9990～0．9996）。该方法应用于市售鲜奶的检测，3种氨基糖苷类抗生素古白回收率为850％～985％．A new method was developed for detection of aminoglycoside antibiotics of tobramycin, neomycin and sisomyein by ion-exchange column with pulsed integrated amperometrie detection. The method was simple enough with the mobile phase of NaOH/H2O and a good detect waveform based on experiments. The detection limits for tobramyein, neomycin and sisomyein were 8, 5, 47 ng/mL respectively ; the linear range was 0.1 - 100 μg/mL （ r = 0. 9990 0. 9995）. The method was applied to the detection of milk samples and spiked samples with recoveries of 85.0 % 98.5 % for the three antibiotics.

关 键 词：离子交换 氨基糖苷类抗生素 妥布霉素 新霉素 西梭霉素 

分 类 号：O657.1[理学—分析化学]