铅诱导的PC12细胞凋亡及caspase-3的激活  

Lead acetate induced apoptosis and the activation of caspase-3 in PC12 cell

作　　者：徐进[1] 季林丹[2] 徐立红[2] 

机构地区：[1]浙江大学劳动卫生与环境卫生研究所,杭州310031 [2]浙江大学医学院生物化学与分子生物学教研室,杭州310031

出　　处：《环境科学学报》2006年第7期1172-1174,共3页Acta Scientiae Circumstantiae

基　　金：国家自然科学基金重点项目(No.20137010);国家自然科学基金面上项目(No.30270257)~~

摘　　要：用MTT法及TUNEL法检测醋酸铅对PC12细胞活力和凋亡率的影响,并用荧光标记的特异性caspase-3抑制剂来测量caspase-3的激活.结果显示,PC12细胞暴露于浓度为0.1、1、10、100μmol·L-1的醋酸铅中24h后,凋亡率和caspase-3的激活显著增加,并具有剂量-效应关系;当铅浓度达到1μmo·lL-1及以上时,细胞活力明显下降.因此可以推论,铅能够诱导PC12细胞发生凋亡,caspase-3的激活可能在这过程中起了非常重要的作用.MTT assay and TUNEL assay were used to detect cell viability and apoptosis rate in PC12 cells respectively. The activation of caspase-3 was measured by fluorescent dye labeled specific inhibitor of caspase-3. The results showed that, both apoptosis rate and activation of caspase-3 in PC12 ceils increased in a dose-dependent manner, after exposure to 0. 1, 1, 10, 100 μ·mol＂ L^-1 lead acetate for 24 h. When the concentration reached 1 μmol· L^-1, cell viability decreased significantly. Therefore, it might be concluded that, lead acetate can induce apoptosis in PC12 cells and the activation of caspase-3 may play an important role in this process.

关 键 词：醋酸铅 细胞凋亡 CASPASE-3 PC12细胞 

分 类 号：R994.6[医药卫生—毒理学]