石柱黄连指纹图谱研究  

Fingerprints of Rhizoma Coptidis from Shizhu by HPLC

作　　者：黄小平[1] 李隆云[1] 瞿显有[1] 崔广林[1] 

机构地区：[1]重庆市中药研究院,重庆400065

出　　处：《中药材》2006年第7期666-669,共4页Journal of Chinese Medicinal Materials

基　　金：国家科技部攻关计划2004BA604A04

摘　　要：目的:建立石柱道地药材黄连的HPLC指纹图谱,为科学评价与有效控制黄连质量提供新方法。方法:RP-HPLC法测定石柱GAP基地10批黄连样品。色谱条件:迪马D iamonsil C18色谱柱,乙腈-0.05 mol/L磷酸二氢钾(磷酸调pH值3)为流动相进行梯度洗脱,检测波长270 nm,流速0.8 m l/m in,柱温25℃。结果:10批黄连样品得到的指纹图谱有16个共有峰,通过与对照品的保留时间及紫外光谱比较,11,12,13,14,15号峰分别为伪小檗碱、药根碱、黄连碱、巴马汀和小檗碱。结论:建立的HPLC指纹图谱分析法能较好反映黄连中的主要生物碱成分,可用于黄连的质量控制。Objective ： To establish a sensitive and specific HPLC method for the quality control of Rhizoma Coptidis collected from shizhu in chongqing. Methods： The HPLC fingerprints of Rhizoma Coptidis from shizhu were obtained from Waters instrument. The methods was performed on a Diamonsil C18 column gradient eluted with acetonitrile-0. 05 mol/L KH2 PO4 （pH3 with H3 PO4 ） at the flow rate of 0. 8 ml/min. The temperature of column was 25℃ and the UV detection wavelength was 270nm. Results ： The HPLC fingerprint of Rhizoma Coptis, showing 16 characteristic peaks, was established from 10 lots of Rhizoma Coptis. By comparision of the retention time and the on-line UV spectra of chemical standards, peak 11,12,13,14 and 15 were identified as Epiberberine, Jatrorrhizine, Coptisine, Palmatine and Berberine. Conclusion ： The HPLC fingerprint of Rhizoma Coptidis with high specificity can be used to control its quality

关 键 词：黄连 高效液相色谱法 指纹图谱 

分 类 号：R282.71[医药卫生—中药学]