甘草抗病毒有效部位(GD4)体外抑制呼吸道合胞病毒作用的研究  

The Anti-Respiratory Syncytial Virus Effect of Active Compound of Glycyrrhiza GD4 in Vitro

作　　者：王秀琴[1] 李洪源[1] 刘鑫妍[1] 张凤梅[1] 李霞[1] 朴英爱[2] 谢志平[1] 陈振华[1] 李鑫[1] 

机构地区：[1]哈尔滨医科大学公共卫生学院,黑龙江哈尔滨150081 [2]黑龙江省药品检验所,黑龙江哈尔滨150001

出　　处：《中药材》2006年第7期692-694,共3页Journal of Chinese Medicinal Materials

摘　　要：目的:观察甘草抗病毒有效部位(GD4)抑制呼吸道合胞病毒(RSV)作用。方法:采用煎煮法、二氯甲烷萃取法和柱层析法提取分离得到甘草抗病毒有效部位(GD4)。采用细胞病变抑制实验,观察GD4在Hela细胞中对RSV的抑制作用。结果:GD4半数中毒浓度(TC50)为0.23 mg/m l,抑制RSV的半数有效浓度(EC50)为28.73μg/m l,治疗指数(TI)为8.0;GD4对RSV的抑制作用存在量效关系;在感染后至少12 h以内给药,GD4能够有效地抑制RSV的复制。GD4不含有甘草酸。结论:甘草抗病毒有效部位(GD4)在Hela细胞中对呼吸道合胞病毒有明显的抑制作用。Objective： To study the effect on anti-respiratory syncytial virus of an active compound GD4 from Glycyrrhiza in vitro. Methods： GD4 was extracted from Glycyrrhiza by hot water extraction, dichloromethane extraction and column chromatography. The CPE inhibition assay was used to test the antiviral activity of GD4 on RSV in Hela cells. Results ： GD4 was effective antiviral agent for RSV in a concentration-dependent manner and its median toxic concentration（TC50 ） and median effeicacious concentration（ EC50 ） and treatment index（TI） was 0. 23 mg/ml, 28. 73μg/ml and 8.0. 120μg/ml GD4 exhibited inhibitory effect when added into cell culture for every 2h within 12h post-infection. GD4 didnt contain glycyrrhizic acid. Conclusion： The active compound of Glycyrrhiza（GD4） was an evident inhibitory effect on RSV.

关 键 词：甘草 呼吸道合胞病毒 抗病毒作用 

分 类 号：R285.5[医药卫生—中药学]