建立评价香加皮质量的RP-HPLC色谱指纹图谱分析方法  

Establishment of RP-HPLC Fingerprint for Quality Assessment of Cortex Periplocae

作　　者：李丹毅[1] 王婷婷[1] 陈晓辉[1] 毕开顺[1] 

机构地区：[1]沈阳药科大学药学院,辽宁沈阳110016

出　　处：《中药材》2006年第7期715-717,共3页Journal of Chinese Medicinal Materials

摘　　要：目的:建立评价香加皮质量优劣的指纹图谱分析方法。方法:以香加皮药材为研究对象,应用RP-HPLC法,色谱柱为Krom asil C18(250 mm×4.6 mm,5μm),乙腈-0.025%磷酸水二元梯度洗脱模式,流速为1.0 m l/m in,检测波长为220 nm,建立了15批不同产地香加皮样品的RP-HPLC色谱指纹图谱。结果:对各产地药材指纹图谱进行聚类分析,根据聚类分析相似度分析结果,将香加皮药材分为4类,选定其中6批优质样品建立共有模式,并应用“中药色谱指纹图谱相似度评价系统2004版”对各产地药材进行了质量评价。结论:该方法简便、可靠,为有效控制香加皮药材质量提供依据。Objective ： To establish the RP-HPLC fingerprint analysis for the quality control of Cortex Periplocae. Methods ： HPLC fingerprint and analysis method of Cortex Periplocae were studied. Kromasil C18 column（250 mm×4.6 mm,5μm） was used, with mixture of acetonitrile and 0.025% phosphate solution as mobile phase in a gradient mode. The flow rate was 1.0 ml/min. The wavelength of measurement was 220 nm. 15 Batches of Cortex Periplocae were determined. Results： The 15 samples were classified as 4 clusters by cluster analysis and the 6 superior in producing area samples were confirmed to establish the mutual model. The samples＇ quality was assessed by“ Similarity Evaluation System for Chromatographic Fingerprint of TCM 2004”. Conclusion： The method is simple and reliable. It can be used to evaluate and control the quality of Cortex Periplocae conveniently.

关 键 词：香加皮 RP-HPLC 指纹图谱 

分 类 号：R286.6[医药卫生—中药学] R286.02[医药卫生—中医学]