大青叶中靛玉红的提取工艺研究  

Study on the Extraction of Indirubin from Isatis indigotica Fort

作　　者：余陈欢[1] 吴巧凤[1] 盛振华[1] 曾晓艳[1] 刘丹华[1] 

机构地区：[1]浙江中医学院药学系,浙江杭州310053

出　　处：《中药材》2006年第7期721-723,共3页Journal of Chinese Medicinal Materials

基　　金：浙江省分析测试基金项目

摘　　要：目的:优选大青叶中靛玉红的最佳提取工艺条件。方法:以靛玉红含量为指标,采用正交实验设计法,考察超声法、回流法不同因素和水平对其提取条件的影响,优选最佳提取工艺。并比较超声法、回流法与传统工艺对靛玉红提取率的影响。结果:三种提取方法中,超声法提取率最高且方法简便。其最佳工艺条件为A1B1C2D3。即加10倍量60%的乙醇、超声提取3次,每次20 m in。结论:超声法具有时间短、收率高、耗能小、无需加热等优点,应用前景广阔。Objective ： To study the optimal process of the extraction of indirubin from Isatis indigotica Fort. Methods ： The process was studied by supersonic extraction, refluxing and orthogonal design with the content of indirubin as the detective marker. Then the extraction of indirubin with supersonic extraction and other methods were compared basing on the yield of extracts. Results： Among them, the supersonic extraction was the simplest and the most rapid and the most complete in extraction. And the optimal conditions were A1B1C2D3 ： supersonic extraction with 60% ethanol, 1 hour, 10-fold solvent and 3 times. Conclusion： The supersonic extraction can extract more indirubin from Isatis indigotica Fort in shorter time with less energy. It also shows a promising prospect for leaching the effective constituents from Chinese herbal medicine by supersonic extraction.

关 键 词：大青叶 靛玉红 提取工艺 正交实验 

分 类 号：R284.2[医药卫生—中药学]