维生素D受体基因多态性与乙型肝炎病毒感染的关联研究  

Study on association between vitamin D receptor gene polymorphisms and the outcomes of HBV infection

作　　者：李俊红[1] 陈冬梅[2] 李卓[1] 刘英[2] 高冀荣[1] 曾宪嘉[2] 钟崇芳[1] 朱席林[2] 勾春燕[1] 潘利[2] 单晶[1] 郭新会[1] 李辉[2] 

机构地区：[1]首都医科大学附属北京佑安医院肝炎研究所,北京100054 [2]中国医学科学院基础医学研究所中国协和医科大学基础医学院流行病学教研室医学分子生物学国家重点实验室

出　　处：《中华医学遗传学杂志》2006年第4期402-405,共4页Chinese Journal of Medical Genetics

基　　金：北京市科委重大项目(H020920020590)

摘　　要：目的探讨中国汉族人群维生素D受体(vitaminDreceptor,VDR)基因多态性是否与乙型肝炎病毒(hepatitisBvirus,HBV)感染结局相关联。方法以184例慢性乙肝患者和205名无症状HBV携带者为研究对象,收集外周血,提取基因组DNA,应用聚合酶链反应-限制性片段长度多态性(polymerasechainreaction-restrictionfragmentlengthpolymorphism,PCR-RFLP)方法,分析VDR基因第2外显子FokⅠ位点、第9外显子TaqⅠ位点的多态性分布。结果单因素分析结果显示FokⅠ位点FF基因型在慢性乙肝组的频率44.6%显著高于无症状HBV携带组的31.7%(P<0.05)。经多因素非条件Logistic回归分析调整混杂作用后,结果仍然显示FF基因型在慢性乙肝组与无症状HBV携带组之间的差异存在统计学意义(OR=1.95,P<0·05)。FokⅠ位点与TaqⅠ位点组成的FT单倍型在慢性乙肝组的分布频率显著高于无症状HBV携带组(OR=1.45,P<0.05),fT单倍型在慢性乙肝组的分布频率显著低于无症状HBV携带组(OR=0.72,P<0.05)。结论维生素D受体基因多态性可能影响HBV感染的遗传易感性。Objective To explore whether the vitamin D receptor gene （VDR） polymorphisms are associated with the outcomes of hepatitis B virus （HBV） infection in Chinese Hart population. Methods Polymerase chain reaction-restr/ction fragment length polymorphism （PCR-RFLP） was used to detect the polymorphisms of Fok Ⅰ locus in exon 2 and Taq Ⅰ locus in exon 9 of VDR gene. One hundred and eighty-four chronic hepatitis B patients and 205 asymptomatic HBV carriers were recruited to make the comparision of frequencies of genotype and haplotype of the VDR gene between the patients and the carriers. Results The univariate analysis showed a significant difference in Fok Ⅰ polymorphism between chronic hepatitis B patients group and asymptomatic HBV carriers group. The FF genotype frequency in chronic hepatitis B patients group was 44.6%, higher than 31.7 % in asymptomatic HBV carriers group （ P 〈 0.05）. After adjusting the confounders by multiple logistic regression analysis, the result still showed a significant difference in Fok Ⅰ site polymorphism between chronic hepatitis B patients group and asymptomatic HBV carriers group （OR = 1.95, P 〈 0. 05）. The FT haplotype frequency in chronic hepatitis B patients group was higher than that in asymptomatic HBV carriers group （ OR = 1.45, P 〈 0.05）. The fT haplotype frequency in chronic hepatitis B patients group was lower than that in asymptomatic HBV carriers group （OR = 0.72, P 〈 0.05）. Conclusion VDR gene polymorphism may be an influence factor of genetic susceptibility to HBV infection.

关 键 词：维生素D受体 乙型肝炎 遗传多态性 

分 类 号：R512.62[医药卫生—内科学]