小鼠PTA1(CD226)胞膜外两个结构域同时与小鼠Tage4(CD155)的相互作用  

Two extracellular Ig-like domains of mouse PTA1(CD226) involved in the interaction with its ligand mouse Tage4(CD155)

作　　者：徐向升[1] 张新海[1] 庄然[1] 金伯泉[1] 

机构地区：[1]第四军医大学基础部免疫学教研室,陕西西安710033

出　　处：《第四军医大学学报》2006年第15期1353-1356,共4页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金重点项目(30030130)

摘　　要：目的:确定mPTA1分子与mTage4参与相互作用的结构域,以深入研究这两种分子的功能.方法:扩增编码mTage4全长分子和mPTA1,hICAM-1分子不同结构域的DNA序列,构建含mTage4,mPTA1不同结构域编码序列的截短体、mPTA1/hICAM-1荧光蛋白嵌合体分子基因的真核表达载体,并转染COS-7真核细胞,通过激光共聚焦扫描显微镜研究这两种分子间的相互作用.结果:DNA序列测定证实成功构建了含mTage4全长分子和mPTA1截短体、mPTA1/hICAM-1嵌合体分子基因的真核表达载体,激光共聚焦扫描显微镜观察结果表明mPTA1截短体分子和mPTA1/hICAM-1嵌合体分子不能与mTage4有效结合,而mPTA1全长分子能与mTage4相互作用.结论:mPTA1分子胞膜外区两个结构域同时参与了与mTage4的相互作用.AIM： To identify the domains of mPTA1 that interact with mouse Tage4 and to study their functions further. METHODS： The DNA fragments encoding mTage4, various domains of rnPTA1 and human ICAM-1 were amplified by PCR and cloned into the eukaryotic expression vector pDsRed2-N1 or pEGFP-N1 respectively, then transfected into eukaryotie COS-7 cells. Confocal laser scanning microscopy was used to confirm the interaction between mPTA1 and mTage4. RESULTS： DNA sequencing confirmed that eukaryotic expression vectors containing mTage4, various domains of mPTA1 or mPTA1/hICAM-1 chimera were constructed successfully. Confocal laser scanning microscopy indicated that mPTA1 truncation and mPTA1/hICAM-1 chimera didn＇t interact with mTage4, whereas full-length mPTA1 did. CONCLUSION： Both extracellular Ig-like domains of mPTA1 are involved in the interaction with its ligand mTage4.

关 键 词：PTA1 CD226 Tage4 CD155 截短体 嵌合体 结构域 

分 类 号：R392.11[医药卫生—免疫学]