肝动脉缺血对犬肝细胞损害的实验研究  

Study of hepatic cellular injury due to hepatic ischemia in dogs

作　　者：蒋基令[1] 杨文军[2] 熊国祚[2] 许志杰[2] 何葵[2] 姜德诵[2] 方勇[3] 

机构地区：[1]广东省东莞市人民医院外科,广东东莞523018 [2]南华大学第二附属医院普通外科,湖南衡阳421001 [3]南华大学第二附属医院病理科,湖南衡阳421001

出　　处：《中国普通外科杂志》2006年第7期533-535,共3页China Journal of General Surgery

摘　　要：目的探讨肝动脉缺血对犬肝细胞的损害。方法对2 0只犬进行肝动脉阻断,不阻断门静脉,分别于阻断前、阻断后2 0,4 0,6 0m in取肝组织进行活检,用常规HE染色和BCL-2免疫组化染色并进行灰度测定。结果肝动脉阻断2 0m in,肝细胞已有明显损害,阻断6 0m in有不可逆性严重损害,BCL-2免疫组化灰度测定,阻断前与阻断后各时点差异均有高度显著性(P<0.0 0 0 1),其中2 0m in和4 0m in与6 0m in之间差异有高度显著性(P<0.0 0 1)。结论肝动脉阻断2 0m in,肝细胞有明显损害,肝动脉阻断6 0 m in,肝细胞有不可逆性损害。Objective To investigate the ischemic injury of hepatic cell caused by hepatic artery occlusion. Methods The hepatic artery was occluded in 20 dogs via operation, while the portal vein remained patent . Specimens were gained from the right liver at four time points ： before occlusion of the hepatic artery , 20 minutes, 40 minutes and 60 minutes after artery occlusion. Each specimen was examined by HE and BCL-2 by immunohistochemistry. The gray scale of BCL-2 in HE sections was detected. Results Hepatic cellular injury was obvious 20 minutes after occlusion of the hepatic artery. Irreversible hepatic cellular injury was observed 60 minutes after hepatic artery occlusion. The results showed that the gray scale of BCL-2 at every time point after hepatic artery occlusion were significantly different from that before hepatic artery occlusion （ P 〈0. 0001 ）. Besides, the hepatic cell injury and BCL-2 expression at 20 minutes and 40minutes after occlusion was significantly different from that of 60 minutes （ P〈 0. 0001 ） , respectively. Conclusions There was obvious hepatic cellular injury 20 minutes after hepatic artery occlusion, and irreversible hepatic cellular injury occured 60 minutes after hepatic artery occlusion.

关 键 词：肝/血液供给 缺血 肝动脉 肝细胞培养 

分 类 号：R322.47[医药卫生—人体解剖和组织胚胎学] R322.121[医药卫生—基础医学]