不同剂量猪肺表面活性物质对大鼠油酸型急性肺损伤疗效的影响  

Influence of different doses of porcine pulmonary on the therapeutic effects in rats with oleic acid induced acute lung injury

作　　者：孙瑜[1] 黄静霞[2] 王轶群[1] 钟纪根[1] 卢建[1] 

机构地区：[1]第二军医大学基础部病理生理学教研室,上海200433 [2]第二军医大学附属长海医院麻醉科,上海200433

出　　处：《中国危重病急救医学》2006年第8期470-473,F0006,共5页Chinese Critical Care Medicine

基　　金：上海市科委重点基础项目(03JC14002)

摘　　要：目的 探讨不同剂量猪肺表面活性物质（PPS）混悬液对油酸致大鼠急性肺损伤（ALI）的治疗作用及量-效关系。方法56只SD大鼠按随机数字表法分为假手术组、油酸模型组和5个不同剂量PPS治疗组。静脉注入油酸诱发大鼠ALI，30min后治疗组和模型组经气管分别滴入50、80、100、150和200mg／kg PPS和等量生理盐水。实验过程中计数大鼠呼吸频率，测定动脉血气。4h后处死，计算大鼠存活率，观察肺组织形态学改变，并检测肺系数、支气管肺泡灌洗液（BALF）中总蛋白含量和血浆肿瘤坏死因子-α（TNF-α）的浓度。结果与模型组比较，PPS50mg／kg组有减慢呼吸频率、短时间内提高动脉血氧分压（PaO2）的作用，但是并不能明显改善肺损伤；PPS80～200mg／kg组除改善呼吸功能外，大鼠肺毛细血管通透性、肺出血、肺水肿、血浆TNF-α浓度以及大鼠死亡率也均明显降低（P均〈0．05）。显示高剂量PPS（150～200mg／kg）在减轻炎症反应和肺损伤方面具有更好的效果。结论单独应用PPS能明显改善早期油酸型ALI大鼠的呼吸功能，≥80mg／kg的PPS有明显减轻肺损伤的作用，而各剂量之间无量-效关系。Objective To investigate the therapeutic effects and dose-effect relationship of intratracheal instillation of different doses of porcine pulmonary surfactant （PPS） in rats with oleic acid （OA） induced acute lung injury （ALI）. Methods Arterial blood gases and respiratory rate during the experiments, survival rate, lung index, total protein （TP） content in bronchoalveolar lavage fluid （BALF）, tumor necrosis factor -α （TNF -α） level in plasma, light microscopy examination of lung specimens after the experiments were determined and performed in control group, OA （0.2 ml/kg） ＋ saline, OA ＋ PPS 50 mg/kg, OA＋PPS 80 mg/kg, OA＋PPS 100 mg/kg, OA＋PPS 150 mg/kg, OA＋PPS 200 mg/kg groups, respectively. Results In PPS 50 mg/kg group, arterial blood gases were improved and respiratory rate was reduced during the first 2 hours （P〈0.05）. Arterial blood gases and reduced breath rates respiratory rate were not improved in other PPS-treatment groups but 4 hours -survival rate was lowered, lung index was decreased, protein contents in BALF and TNF -α level in serum were lowered, and pathological changes were ameliorated compared with group given saline after OA, especially in high dosage of PPS （150- 200 mg/kg） group （P〈 0.05）. Conclusion Administration of PPS via trachea provides obvious effects on respiratory functions in rats with OA induced ALI, moreover PPS （≥80 mg/kg） alleviates lung injury. There is no dose-effect relationship of PPS in PPS- treatment groups.

关 键 词：油酸 肺损伤 急性 肺表面活性物质 量-效关系 

分 类 号：R563.8[医药卫生—呼吸系统]