胃旁路术对糖尿病大鼠的降糖作用及其机制  

Hypoglycemic effect of gastric bypass on diabetic rats and its mechanisms

作　　者：张士虎[1] 苗毅[1] 

机构地区：[1]南京医科大学第一附属医院普外科,210029

出　　处：《中华实验外科杂志》2006年第8期997-999,共3页Chinese Journal of Experimental Surgery

基　　金：江苏省卫生厅135重点学科基金(苏卫科教[2001]31号)

摘　　要：目的观察胃旁路术(GBP)对链脲佐菌素(STZ)诱发的糖尿病大鼠降糖作用。探讨其机制。方法SD大鼠注射STZ建立糖尿病模型后分为手术组(O组)、假手术组(S组)、饮食控制组(F组)、对照组(C组),每组8只,测术前,术后第1、2、3、4、8周空腹和口服葡萄糖后血糖、胰岛素、胰高血糖素样肽-1(GLP-1)和体重、平均进食量。结果O组GBP后3周,空腹和餐后血糖分别由(16.84±3.82)、(31.88±6.22)mmol/L下降到(13.24±3.53)、(17.35±3.47)mmol/L(P值均＜0.05),空腹和餐后胰岛素分别由(28.66±8.17)、(30.73±8.99)mIU/L上升到(46.48±10.41)、(51.14±11.45)mIU/L(P值均＜0.01)。空腹和餐后GLP-1分别由(7.02±2.10)、(42.20±11.16)pmol/L上升到(25.16±7.30)、(97.83±30.23)pmol/L(P值均＜0.01)。GLP-1和血糖成负相关(P＜0.01),GLP-1和胰岛素成正相关(P＜0.01)。S组体重改变与O组相似,血糖无明显下降；F组控制平均进食量约为O组的1/3并致显著体重下降,血糖下降没有O组明显(P＜0.05)。结论GBP能显著降低STZ大鼠血糖,可能通过术后GLP-1分泌增多起作用。GBP的降糖作用与术后大鼠饮食减少和体重下降无关。Objective To study the hypoglycemic effect of gastric bypass （GBP） on streptozotoein （STZ）-induced diabetic rats and its mechanisms. Methods Thirty-two male SD diabetic rats induced by STZ were randomly allocated into 4 groups： GBP （group O）, sham operation （group S）, food restriction （group F） and controls （group C）. Plasma glucose concentrations, insulin and glucagon-like peptide-1 （GLP-1） were measured respectively at fasting and postprandial condition before and on the 1st, 2nd, 3rd, 4th and 8th week after GBP in all rats, and weight and food intake were measured simultaneously. Results Three weeks after operation in the GBP rats, mean fasting and postprandial plasma glucose levels were declined respectively from （16.84± 3.82）, （31.88± 6.22） to （13.24± 3.53）, （17.35 ± 3.47） mmol/L （P 〈 0.05）, fasting and postprandial plasma insulin levels increased respectively from （28.66± 8.17）, （30.73± 8.99） to （46.48± 10.41）, （51.14± 11.45） mIU/L （P〈0.01）,and fasting and postprandial plasma GLP-1 elevated respectively from （7.02± 2.10）, （42.20 ± 11.16） to （25.16 ± 7.30）, （97.83 ± 30.23） pmol/L （ P 〈 0.01） respectively. These results were not seen in the group S despite similar postoperative weight gain profile. GBP resulted in better glycemic control than greater weight loss from food restriction （P 〈 0.05）. GLP-1 was negatively correlated with plasma glucose （P〈0.01） and positively with insulin （P〈0.01）.Conclusion GBP can markedly reduce plasma glucose in STZ-diabetic rats, and the hypoglycemic effect may be contributed to more GLP-1 secretion after GBP, but not to lower food intake or weight loss.

关 键 词：糖尿病 胰高血糖素样肽-1 治疗 

分 类 号：R587.1[医药卫生—内分泌] R977.15[医药卫生—内科学]