应用碱性成纤维细胞生长因子基因治疗促进大鼠早期断蒂皮瓣的成活  

The effect of basic fibroblast growth factor gene on the survival of flaps with early pedicle division: an experimental study with rat

作　　者：朱华锋[1] 汪春兰[1] 赵宇[1] 曹东升[1] 丁浩[1] 王帮河[1] 王伟[1] 王兴宇[1] 姚远[1] 

机构地区：[1]安徽医科大学第一附属医院整形外科,合肥230022

出　　处：《安徽医科大学学报》2006年第4期386-388,共3页Acta Universitatis Medicinalis Anhui

基　　金：安徽省科技厅自然科学基金资助项目(编号:03023056)

摘　　要：目的探讨腺病毒介导的碱性成纤维细胞生长因子基因(Ad-bFGF)对大鼠随意皮瓣早期断蒂的影响。方法SD♂大鼠,普通级,随机分成3组:Ad-bFGF目的组、Ad-GFP对照组、PBS对照组,每组10只。于大鼠背部设计2cm×6cm随意皮瓣,蒂位于髂脊水平。术后4d断蒂,断蒂后7d观测皮瓣成活面积,检测bFGF表达情况,计算血管密度。结果目的组皮瓣的成活率、血管密度和染色深度均较两对照组有显著差异(P<0.05)。结论应用Ad-bFGF基因治疗能够促进大鼠随意皮瓣早期断蒂。Objective To investigate the effect of locally administered adenovirus-mediated basic fibroblast growth factor （ad-bFGF） on rat flaps with early pedicle division. Methods Thirty male Sprague-Dawley rats were randomly divided into three groups： Ad-bFGF group, Ad-GFP group and PBS group. The expression of bFGF and density of capillary marked with CD34 were examined using morphological and immunohistochemical analysis. Results The flap survival rates of the three groups were （97. 8 ± 2. 5 ） %, （78.3 ± 2.2 ） % and （79.2% ± 2. 3 ） % respectively and the density of capillary sections was （27.5 ±4. 3） /HP, （ 17.6 ±3.1 ） /HP and （ 18.5 ±3.0） / HP. Immunohistochemical staining revealed increased deposition of bFGF in group the Ad-bFGF compared to the other groups. Conclusion The Ad-bFGF gene therapy improves the survival of the flaps with early pedicle division.

关 键 词：成纤维细胞生长因子2/遗传学 基因疗法 外科皮瓣 

分 类 号：R459.9[医药卫生—治疗学] R622.1[医药卫生—临床医学]