间断压力对离体培养成骨细胞作用的研究  

Study of the effect of intermittent compressive pressure to the osteoblasts in vitro.

作　　者：李可心[1] 尚天裕 董福慧 张碧辉[1] 陈楚楚[1] 贝时璋[1] 钱民全 赵笃风 

机构地区：[1]中国中医研究院骨伤科学究所,中国科学院生物物理研究所,中国科学院力学研究所

出　　处：《中国骨伤》1996年第5期7-9,共3页China Journal of Orthopaedics and Traumatology

基　　金：国家自然科学基金

摘　　要：实验模拟骨在功能活动过程中细胞外环境压力的变化这一生理过程，给实验组离体培养成骨细胞提供间断气体压力变化（０．０９８ＭＰａ，１５分钟加压，１５分钟无压力，２周期／小时，每天加压８小时），发现成骨细胞数逐步增加，且碱性磷酸酶活性也明显增高，均与对照组有明显差异。结果提示，间断加压可促进离体培养成骨细胞的增殖与分化。The experiment imitated the physiological changes of the cellular extemal circumstances, existed during skeletal functional movement, and supplied a intermittent compressive pressure(0.098 MPa, 15 minutes pressrue, 15 minutes relax, 2 cycles/one hour, 8 hours/day) to the osteoblasts of experiment al groups in vitro.It was discovered that the numbers of osteoblasts and the reaction of alkaline phosphatase in the experimental group were markedly elevated than that of the control groups. The results indicate that the intermittent compressive prssure is able to improve the proliferation and differentiation of the osteoblasts.

关 键 词：间断压力 成骨细胞 培养 

分 类 号：R681[医药卫生—骨科学]