不同年龄大鼠血清胰岛素样生长因子Ⅱ水平的变化  

Changes of level of insulin-like growth factor Ⅱ in serum of Wistar rats of different ages

作　　者：李艳芳[1] 刘宇扬[1] 梁铁彪[2] 王建龙[1] 成万钧[1] 

机构地区：[1]首都医科大学附属北京安贞医院十二病房,北京100029 [2]北华大学附属医院心内科,吉林吉林132001

出　　处：《中华老年心脑血管病杂志》2006年第8期549-551,共3页Chinese Journal of Geriatric Heart,Brain and Vessel Diseases

基　　金：北京市自然科学基金项目(7042056)

摘　　要：目的研究不同年龄Wistar大鼠血清胰岛素样生长因子Ⅱ(IGF-Ⅱ)、胰岛素(INS)和胰岛素抗体水平的变化。方法采用放射免疫分析法分别测定35只3月龄、12、18和24月龄Wistar大鼠血清IGF-ⅡI、NS和胰岛素抗体水平的变化。结果餐后血清IGF-Ⅱ在3月龄大鼠明显高于12、18和24月龄大鼠(P<0.05,P<0.01,P<0.05)。餐后的INS水平在12、18和24月龄均较3月龄大鼠明显升高(P<0.01)。4组大鼠餐后的胰岛素抗体水平均<5%,为阴性。结论餐后IGF-Ⅱ水平随年龄增长而降低,但在中年以后(12月龄大鼠)则处于稳定水平;餐后的INS水平随年龄增长而升高,中年以后INS也处于稳定水平。Objective To observe changes of levels of insulin-like growth factor Ⅱ （IGF-Ⅱ ）, insulin （INS） and insulin antibody in serum of Wistar rats of different ages. Methods Radioimmunoassay was adopted to measure the levels of IGF-Ⅱ ,INS and insulin-antibody in sera of 3, 12, 18 and 24 month -old Wistar rats （n = 35）. Results The levels of IGF-11 were increased significantly after meal in 3 month-old rats compared with 12, 18 and 24 month-old （ P 〈0.05, P 〈0.01, P 〈0.05）. The levels of INS were elevated after meal in 12, 18 and 24 month-old Wistar rats compared with 3 month-old （ P 〈 0.01）. The levels of insulin antibody were lower than 5 % （negative） in four groups. Conclusions The levels of IGF-Ⅱ decrease with increasing age, but were stable after middle age. The levels of INS increase with increasing age, but were stable after middle age. There might be mutual regulation between IGF-Ⅱ and INS.

关 键 词：胰岛素生长因子Ⅱ 胰岛素抗体 胰岛素 年龄因素 

分 类 号：R33[医药卫生—人体生理学]