去痴灵脂溶性提取物血清抑制M146L细胞分泌β淀粉样蛋白  

Inhibition of liposoluble extracts from Quchiling on M146L cell-secreted amyloid beta-proteins

作　　者：余锐[1] 肖飞[1] 李晓光[1] 罗焕敏[1] 

机构地区：[1]暨南大学药学院神经药理研究室,广州510632

出　　处：《中国新药杂志》2006年第13期1061-1063,共3页Chinese Journal of New Drugs

基　　金：国家自然科学基金(30472014);广州市科技局(200523-E5081)资助

摘　　要：目的:研究中药复方去痴灵脂溶性提取物血清对转染人类β淀粉样前体蛋白(amyloid beta-protein precursor,APP)基因和早老素-1(prsenilin-1,PS-1)基因的M146L细胞分泌β淀粉样蛋白42(amyloid beta-protein 42,Aβ_(42))的抑制作用。方法:采用血清药理学方法,制备去痴灵脂溶性提取物的含药血清。体外培养高效表达Aβ_(42)的M146L细胞株,建立Aβ_(42)过度表达的细胞模型。加入不同浓度(1%,3%和9%)的含药血清,用CCK-8(cell counting kit-8)比色实验检测含药血清对M146L细胞毒作用,用ELISA法(enzyme-linked immunosorbent assay)测定M146L细胞分泌Aβ_(42)的变化。结果:3个浓度的去痴灵脂溶性提取物血清对M146L存活率没有影响,不具有细胞毒作用。3个浓度的含药血清均能显著抑制M146L细胞分泌Aβ_(42),以高浓度组最为明显。结论:一定浓度(1%, 3%,9%)的去痴灵脂溶性提取物血清对M146L细胞分泌Aβ_(42)有明显的抑制作用。Objective： To investigate if Quchiling liposoluble extracts-containing serum （QLS） inhibit the expressions of amyloid beta-protein 42 （Aβ42） by transformed human beta-protein precursor （APP） gene and presenlin-1 gene of Chinese hamster ovary （CHO） ceils （M146L）. Methods： The QLS was isolated from rats by the serum pharmacology. The M146L ceils were cultured together with QLS solutions （1% , 3% or 9% ） to produce considerable A^42 in vitro. A cell counting kit （CCK-8） test was used to assess cell viability. An enzyme-linked immunosorbent assay （ELISA） was performed to analyze Aβ42 secretion. Results ： The CCK-8 test indicated that different concentrations of QLS showed no toxicity towards the cultured M146L cell survival. The ELISA disclosed that the Aβ42 generations by the M146L ceils were suppressed by the QSL in a concentration-dependent manner. Conclusion： The QLS inhibits the M146L cells-secreted Aβ42 by an unclear mechanism.

关 键 词：去痴灵 脂溶性提取物 血清药理学 阿尔茨海默病 Β淀粉样蛋白 

分 类 号：R285.5[医药卫生—中药学] R286.1[医药卫生—中医学]