保肾口服液对IgA肾病小鼠肾小球转化生长因子β_1蛋白及mRNA的影响  

Effect of Protecting Kidney Oral Solution on Transforming Growth Factor β,and mRNA in IgA Nephropathy Rats

作　　者：吴竞[1] 杨爱国[1] 阮诗玮[1] 王智[1] 陈杨荣[1] 洪江淮[1] 丘余良[1] 

机构地区：[1]福建中医学院附属人民医院,福建福州350004

出　　处：《福建中医学院学报》2006年第4期30-32,共3页Journal of Fujian College of Traditional Chinese Medicine

基　　金：福建省卫生厅资助项目(2001-707)

摘　　要：为观察TGFβ-1蛋白及mRNA基因在IgA肾病小鼠肾小球中的表达及保肾口服液对该表达的影响。采用口服牛血清白蛋白及静注葡萄糖球菌肠毒素制成小鼠IgA肾病模型,免疫组化和原位杂交检测肾小球转化生长因子β1(TGFβ-1)蛋白和mRNA。结果5个治疗组中保肾口服液大剂量组肾小球内TGFβ-1蛋白表达最弱;保肾口服液大剂量组比模型组TGFβ-1mRNA表达减弱,其它组没有变化。表明大剂量保肾口服液可能抑制IgA肾病小鼠肾脏局部TGFβ-1基因表达而达到下调肾脏局部TGFβ-1蛋白质的表达水平从而减轻肾损伤,起到保护肾脏的作用。To observe the mRNA and protein expression of TGF-β1 in IgA nephropathy rats and the therapeutic effect of protecting kidney oral solution on it. The IgA nephropathy rats model were made by the method of oral BSA and intravenous SEB, the mRNA and protein expression of TGF-β1 was measured by in-situ hybridisation and immunohistoehemistry. The results showed that the protein expression of TGF-β1 in large dosage group was the less among 5 treatment groups, while mRNA expression decrased. Conclusion： Protecting kidney oral solution may inhibit mRNA and protein exnression of TGF-β1. thus reduce kidney iniury.

关 键 词：保肾口服液 IGA肾病 转化生长因子Β1 免疫组化 原位杂交 

分 类 号：R285[医药卫生—中药学]