用夹心酶联免疫法研究hGM－CSF在大鼠体内的药物代谢动力学  

STUDY ON PHAKMACOKINETICS OF HUMAN GRANULOCYTE-MACKOPHAGE COLONY STIMULATING FACTOR (hGM-CSF)IN RATS USING A SENSITIVE IMMUNOASSAY(ELISA)

作　　者：何小庆[1] 雷小虹[1] 韩锐[1] 

机构地区：[1]中国医学科学院,中国协和医科大学药物研究所

出　　处：《药学学报》1996年第6期406-410,共5页Acta Pharmaceutica Sinica

摘　　要：用灵敏的夹心酶联免疫法（ＥＬＩＳＡ）建立了检测重组人ｈＧＭ－ＣＳＦ药代动力学的方法。此方法ｈＧＭ～ＣＳＦ在１２．５～０．３９ｎｇ·ｍｌ￣（－１）范围内，检测呈线性相关。最低检测灵敏度为０．４ｎｇ·ｍｌ￣（－１）。本方法不受血清中潜在干扰因素的影响，检测具高度特异性。大鼠ｓｃｈＧＭ－ＣＳＦ５０，１００，２００μｇ·ｋｇ￣（－１）后１５ｍｉｎ，血中即有较高浓度的ｈＧＭ－ＣＳＦ，１ｈ左右达峰浓度，以后快速下降。皮下给药，尿中可检测到原型ｈＧＭ－ＣＳＦ，但累积排泄率较低。本研究为临床合理用药提供依据。A sensitive and reliable sandwich enzyme linked immunosobent assay(ELISA)has been developed for determination of concentration of recombinant human granulocyte-macrophagecolony-stimulating factor(hGM-CSF).The assay is quantitative between 0.39～12.5 ng·ml￣(-1)forbacterially synthesized hGM-CSF in rat serum and urine. The methed was shown to be highly specificand did not significantly alter the determination when adding some potential interfering substances.After single sc injection of hGM-CSF 501 100 or 200μg·kg(-1), a high GM-CSF level was detectedabout 1 5 min in rat serum, the highest level of hGM-CSF was two apparent phases with half-livesT_(1/2α) of 0.72,0.70,0. 80 h and T_(1/2β) of 8. 77,8. 87 and 5. 58h.A detectable urinary excretionoccured after sc injection of hGM-CSF 200μg· kg(-1),but the total urinary excretion of unchangedhGM-CSF was verv low.

关 键 词：HGM-CSF 药代动力学 ELISA 

分 类 号：R977.6[医药卫生—药品] R979.5[医药卫生—药学]